CRISPR/dCas9 Activation Plasmids consist of three plasmids at a 1:1:1 mass ratio. The second plasmid encodes the MS2-p65-HSF1 fusion protein, and a hygromycin resistance gene.
Cre VectorCRISPR基因敲除和激活产品(): sc-418923
4.5(2)
寫評論提問- 20 µg纯化即用型质粒DNA，最多可供20次转染
- Cre Vector表达Cre重组酶，催化两个LoxP位点之间的位点特异性DNA重组
- Cre 载体的设计目的是专门针对 HDR 质粒插入的选择标记侧翼的 LoxP 位点，并切除遗传物质
- Cre Vector含有一个CMV启动子，以启动Cre重组酶的表达
- 转染后，可以用针对目的基因的抗体，通过WB、IF或IHC分析细胞；或继续转染，做其他DNA编辑
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说明书与实验方案
订购信息
| 产品名称 | 产品编号 | 规格 | 价格 | 数量 | 收藏夹 | |
Cre载体 | sc-418923 | 20 µg | CNY624.00 |
do you have any educational information
Asked by: Gary
For assistance please contact Technical service . Please call 800-457-3801 or email [email protected].
Answered by: BlakeJ
Date published: 2026-01-21
Hi, can the above plasmid be used for yeast namely saccharomyces cerevisiae?
Asked by: NT777
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Answered by: lai13
Date published: 2024-07-25
How to transfect the cre-vector to the cells that already have puromycin gene incorporated by HDR vector? transfect, then wait two or three days and consider those cells puromycin free? correct? do I select?
Asked by: michaela
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Answered by: lai13
Date published: 2024-07-25
Can you provide the plasmid map and size of the vector? Thanks,
Asked by: Chris1
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Answered by: lai13
Date published: 2024-07-25
Does this kit includes a vector to flanked my target gene?
Asked by: Ldas01
Thank you for your question. It would be helpful if you could call us directly at 800-457-3801 ext.1, allowing for a more interactive discussion of this and other related questions.
Answered by: Blake J
Date published: 2022-01-26
How large is this plasmid?
Asked by: Dkr0
Thank you for your question. Our Cre Vector sc-418923 has a size of approximately 5.9 kilobases.
Answered by: Tech Support Europe
Date published: 2024-04-20
Why is CRE Vector Transfection "Recommended" after for CRISPR/Cas9 KO Plasmid and HDR Plasmid Transfection (following selection)? Is it simply to remove the selection marker? Or, does it help in any way with knocking out the gene?
Asked by: jmd6207
Thank you for your question. It is not mandatory, and only recommended if you wish to removed the puromycin selection marker. Removing the HDR plasmid insert using the CRE vector does not help with knocking out the gene. It is useful if you wish you knock out a second gene or if you need you cells to no longer be puromycin resistant.
Answered by: Tech Service
Date published: 2024-03-19
whats the cre loxp systems
Asked by: waleed xel
Thank you for your question. The Cre-Lox system is a site-specific recombinase technology, used to carry out deletions, insertions, translocations and inversions at specific sites in the DNA of cells. You can find our more about the Cre-Lox system at any of the links below:
https://en.wikipedia.org/wiki/Cre-Lox_recombination
https://www.youtube.com/watch?v=oLPjiwM0G7A&app=desktop
Answered by: Tech Service
Date published: 2022-05-04
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Rated 5 out of
5 by
Jelena from
Very useful product.Very useful tool to achieve double or triple knock-out with the CRISPR system since the most of Santa Cruz CRISPR products are resistant to the same antibiotic-pyromycin.
Date published: 2017-04-25
Rated 4 out of
5 by
mask from
Remove the marker gene efficientlyI used this vector to remove the neo marker-gene in the pig primary cells . The marker-free efficiency was so high to 80% .I nucleofected the 4ug Cre vector to the 1000000 cells by ALONZO. But, i suggested that can the CAG promoter may replace the CMV promoter of this vector, because of the CMV promoter may Cause gene-silencing in some special cell lines
Date published: 2016-11-21
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Cre VectorCRISPR基因敲除和激活产品() is rated
4.5 out of
5 by
2.
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版权所有© 2007-2026 Santa Cruz Biotechnology, Inc. 保留所有权利。”Santa Cruz Biotechnology” 和Santa Cruz Biotechnology, Inc.的商标，”Santa Cruz Animal Health”, “San Juan Ranch”, “Supplement of Champions”, San Juan Ranch的商标，”UltraCruz”, “ChemCruz”, “ImmunoCruz”, “ExactaCruz”, 和 “EZ Touch”是Santa Cruz Biotechnology, Inc.的注册商标。所有商标均为其各自所有者的财产。