Date published: 2025-9-6
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CD45 Antibody (35-Z6) - Western Blotting - Image 384065
CD45 抗体 (35-Z6) HRP: sc-1178 HRP. 对 Jurkat (A), CCRF-CEM (B), BJAB (C), Ramos (D) 和 NAMALWA (E) 全细胞裂解液中 CD45 表达的直接 Western 印迹分析.
CD45 抗体 (35-Z6) HRP: sc-1178 HRP. 对 Jurkat (A), CCRF-CEM (B), BJAB (C), Ramos (D) 和 NAMALWA (E) 全细胞裂解液中 CD45 表达的直接 Western 印迹分析.

CD45 抗体 (35-Z6): sc-1178

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说明书
  • CD45 抗体 35-Z6 是小鼠单克隆 IgG1 κ，CD45抗体, 在98篇文献中引用，规格为200 µg/ml
  • 针对CD45的胞外结构域，源自human
  • CD45 抗体 (35-Z6) 推荐用于 WB, IP, IF, IHC(P) 和 FCM，检测mouse, rat 和human 来源的 CD45
  • 抗CD45抗体（35-Z6）可与琼脂糖结合用于IP；与HRP结合用于WB、IHC(P)和ELISA；与藻红蛋白或FITC结合用于IF、IHC(P)和FCM
  • 还可偶联Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 和 Alexa Fluor® 647，用于WB (RGB), IF, IHC(P) 和 FCM, 以及用于RGB荧光成像系统，例如iBright™ FL1000, FluorChem™, Typhoon, Azure和其他类似的系统
  • 还可偶联Alexa Fluor® 680 和 Alexa Fluor® 790， 用于WB (NIR), IF 和 FCM; 以及用于近红外（NIR）检测系统，如LI-COR®/Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure和类似系统
  • 还可偶联Alexa Fluor® 405 用于IF, IHC(P) 和FCM
  • 关于如何获取CD45 (35-Z6): sc-1178的免费10 µg小样，联系我们技术服务部门 (或者您当地的代理商)了解详情。
  • m-IgG Fc BP-HRP 和 m-IgG1 BP-HRP 是 CD45 抗体 (35-Z6) 在 WB and IHC(P) 应用中的首选二抗检测试剂。这些试剂现与CD45 抗体 (35-Z6) 组合成套装提供。 (请参阅下面的订购信息)。

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描述
基因信息
说明书与实验方案
研究信息

関連項目

此外 CD45 抗体包括 CD45R, CD45RA, CD45RB, CD45RC 和 CD45RO

CD45抗体（35-Z6）是一种小鼠单克隆IgG1 kappa轻链抗体，用于通过蛋白质印迹（WB）、免疫沉淀（IP）、PAR检测和流式细胞术（FCM）在小鼠、大鼠和人类样本中检测CD45（也称为蛋白酪氨酸磷酸酶受体C型（PTPRC））。抗CD45抗体（35-Z6）靶向人类CD45的胞外结构域，确保在多种物种间实现精确识别。CD45是一种重要的跨膜糖蛋白，广泛表达于造血细胞，在调节免疫细胞信号传导方面发挥着重要作用。CD45作为磷酪氨酸磷酸酶，可调节Src和Syk家族激酶（如Lck、Fyn和ZAP-70）的活性，这些激酶对于T和B细胞受体结合后启动信号级联至关重要。这种调节对于有效的T细胞激活、增殖和分化以及B细胞发育和抗体产生至关重要。CD45的不同异构体由外显子4、5和6的替代剪接产生，可对免疫反应进行微调，以适应特定细胞类型和激活阶段。CD45失调与多种免疫疾病有关，包括自身免疫性疾病和白血病，这凸显了其在维持免疫稳态方面的重要性，以及作为治疗靶标的潜力。CD45 (35-Z6) 抗体有非结合型和多种结合型，如琼脂糖、辣根过氧化物酶 (HRP)、藻红蛋白 (PE)、异硫氰酸荧光素 (FITC) 和 Alexa Fluor® 结合物，为各种实验应用提供了多种选择。抗CD45抗体（35-Z6）可有效研究CD45的表达、同型异构体分布和功能动态，从而增进我们对免疫细胞生物学和相关疾病机制的理解。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

CD45 抗体 (35-Z6) 参考文献:

  1. CD45蛋白-酪氨酸磷酸酶抑制剂的开发。  |  Lee, K. and Burke, TR. 2003. Curr Top Med Chem. 3: 797-807. PMID: 12678845
  2. CD45：免疫调节的直接和间接调控。  |  Huntington, ND. and Tarlinton, DM. 2004. Immunol Lett. 94: 167-74. PMID: 15275963
  3. CD45糖基化控制T细胞的生死。  |  Earl, LA. and Baum, LG. 2008. Immunol Cell Biol. 86: 608-15. PMID: 18607388
  4. 细胞表达受限的 CD45 分子中依赖于 Sialic acid 的表位。  |  Bazil, V., et al. 1989. Immunogenetics. 29: 202-5. PMID: 2466778
  5. 鉴定与 B220 和其他 T200 限制性抗体反应所需的小鼠 CD45 (T200) 替代剪接外显子。  |  Johnson, P., et al. 1989. J Exp Med. 169: 1179-84. PMID: 2466938
  6. 三个外显子的不同用法产生了至少五种不同的 mRNA，编码人类白细胞共同抗原。  |  Streuli, M., et al. 1987. J Exp Med. 166: 1548-66. PMID: 2824653
  7. 石蜡包埋组织切片中与白细胞亚群反应的单克隆抗体（MT1, MT2, MB1, MB2, MB3）。  |  Poppema, S., et al. 1987. Am J Pathol. 127: 418-29. PMID: 3296769
  8. 在石蜡切片中显示 B 细胞, T 细胞和骨髓抗原。  |  West, KP., et al. 1986. J Pathol. 150: 89-101. PMID: 3540244
  9. B 淋巴细胞的新标记 MB2：与传统组织切片中活跃的其他淋巴细胞亚群标记比较。  |  Hall, PA., et al. 1987. J Clin Pathol. 40: 151-6. PMID: 3546393
  10. 蛋白酪氨酸磷酸酶对免疫功能的调节。  |  Okumura, M. and Thomas, ML. 1995. Curr Opin Immunol. 7: 312-9. PMID: 7546394
  11. 产生针对小鼠淋巴细胞表面糖蛋白 T200 单克隆抗体的种间脾脏-骨髓瘤杂交种。  |  Trowbridge, IS. 1978. J Exp Med. 148: 313-23. PMID: 78961
  12. CD45：作为淋巴细胞活化和发育所需的蛋白酪氨酸磷酸酶的新角色。  |  Trowbridge, IS. and Thomas, ML. 1994. Annu Rev Immunol. 12: 85-116. PMID: 8011300

订购信息

产品名称产品编号规格价格数量收藏夹

CD45 抗体 (35-Z6)

sc-1178
200 µg/ml
RMB2377.00

CD45 (35-Z6): m-IgG Fc BP-HRP 套装

sc-526464
200 µg Ab; 10 µg BP
RMB2662.00

CD45 (35-Z6): m-IgG1 BP-HRP 套装

sc-531837
200 µg Ab; 20 µg BP
RMB2662.00

CD45 抗体 (35-Z6) AC

sc-1178 AC
500 µg/ml, 25% agarose
RMB3129.00

CD45 抗体 (35-Z6) HRP

sc-1178 HRP
200 µg/ml
RMB2377.00

CD45 抗体 (35-Z6) FITC

sc-1178 FITC
200 µg/ml
RMB2482.00

CD45 抗体 (35-Z6) PE

sc-1178 PE
200 µg/ml
RMB2580.00

CD45 抗体 (35-Z6) Alexa Fluor® 488

sc-1178 AF488
200 µg/ml
RMB2685.00

CD45 抗体 (35-Z6) Alexa Fluor® 546

sc-1178 AF546
200 µg/ml
RMB2685.00

CD45 抗体 (35-Z6) Alexa Fluor® 594

sc-1178 AF594
200 µg/ml
RMB2685.00

CD45 抗体 (35-Z6) Alexa Fluor® 647

sc-1178 AF647
200 µg/ml
RMB2685.00

CD45 抗体 (35-Z6) Alexa Fluor® 680

sc-1178 AF680
200 µg/ml
RMB2685.00

CD45 抗体 (35-Z6) Alexa Fluor® 790

sc-1178 AF790
200 µg/ml
RMB2685.00

CD45 抗体 (35-Z6) Alexa Fluor® 405

sc-1178 AF405
200 µg/ml
RMB2685.00

Can this antibody detect CD45 in Zebrafish?

Asked by: Becky
CD45 has only been validated for Mouse, Rat, and Human. CD45 has no similar homology to zebrafish.
Answered by: Technical Service
Date published: 2023-10-10

Does this antibody detect WBC from C57Bl/6 mice?

Asked by: frneubh1
Thank you for your question. Anti-CD45 Antibody (35-Z6) is recommended for detection of CD45 of mouse, rat and human origin by WB, IP, IF, IHC(P) and FCM
Answered by: Tech Support Europe
Date published: 2021-04-14

If I am going to stain mouse skin tissues, do I need additional kit, for example "mouse on mouse kit" (as this antibody is mouse origin)?

Asked by: hpark
Thank you for your question. It depends on what type of tissues you are staining. If you are staining mouse skin tissues, and there is not a lot of endogenous mouse IgG, then it is probably not necessary to use a special "mouse on mouse kit" for staining. We do recommend including a negative control tissue where you omit primary antibody and stain with secondary antibody alone. Then you can identify any possible non-specific binding from your anti-mouse IgG secondary antibody alone. As an alternative, you could also consider using one of the directly conjugated forms available of this CD45 antibody. Then no secondary antibody is needed for detection, and you can avoid any possible problem of non-specific binding from anti-mouse IgG secondary antibodies.
Answered by: Technical Support
Date published: 2020-05-18

What could be the expiry date for CD45 since we have observed it to be active even after 5 years

Asked by: Samarth
Thank you for your question. We do not give our antibodies an expiration date since they can work for up to 10-15 years in some cases. We warranty the performance of our antibody reagents for 1 year from the date of purchase.
Answered by: Tech Service
Date published: 2020-02-26

I am using the LiCor/Odyssey system. Which secondary antibody do you recommend using with CD45 Antibody (35-Z6): sc-1178?

Asked by: jenni
We recommend using one of our our exclusive Mouse IgG Binding Proteins (m-IgGκ) conjugated to CFL 680 or CFL 790. They are suitable for use with Near-Infrared (NIR) detection systems, such as LI-COR/Odyssey and other comparable systems. A complete list of available binding proteins is available on our website here: https://www.scbt.com/scbt/browse/support-products-mouse-igg-binding-proteins/_/N-ecrety
Answered by: Technical Support 6
Date published: 2017-01-09
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Rated 4 out of 5 by from Good for WBProvides a good signal for WB (tested in human PBMC samples).
Date published: 2023-10-15
Rated 4 out of 5 by from WBWe tested this antibody in WB and we obtained good results
Date published: 2022-06-21
Rated 5 out of 5 by from It works!We have tried this Ab in Western Blot and it works fine at the dilution of 1:200.
Date published: 2022-04-06
Rated 5 out of 5 by from Excellent antibody for IFWe use the antibody to stain T cells in a coculture assays with patient derived prostate cancer organoids. 1:400 dilution in 1% BSA (shown in red).
Date published: 2021-03-11
Rated 5 out of 5 by from Great CD45 Ab for DAB immunohistochemistryThe Ab gives strong and clean signal/noise staining of CD45 in FFPE colon tissue from mouse
Date published: 2019-09-20
Rated 5 out of 5 by from immunofluorescent stainingon immunofluorescent staining this sc-1178 CD45 antibody worked well.
Date published: 2019-04-16
Rated 4 out of 5 by from Consistent QualityWe continue to use this antibody for IF and flow, though have not had success with IP.
Date published: 2019-03-18
Rated 1 out of 5 by from Bad result on mouse samplesBad result on mouse bone marrow paraffin samples. Few cells stained.
Date published: 2018-03-15
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CD45 抗体 (35-Z6) is rated 4.3 out of 5 by 30.
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