CD300A 抗体 (MEM-260): sc-101318

CD300A Antibody (MEM-260) is an IgG₁ mouse monoclonal CD300A antibody (also designated CD300A antibody) that detects the CD300A protein of human origin by WB, IP and FCM. CD300A Antibody (MEM-260) is available as both the non-conjugated anti-CD300A antibody form, as well as multiple conjugated forms of anti-CD300A antibody, including PE. CD300A, also known as CMRF35H, IRC1, IRC2, IRp60 or IGSF12, is a 299 amino acid single-pass type I membrane protein that localizes to the cell membrane and contains one Ig-like V-type domain. Expressed by mast cells, B-cells, dendritic cells, monocytes, granulocytes and natural killer (NK) cells, CD300A functions as an inhibitory receptor that is thought to help down-regulate the cytolytic activity in NK cells and may also down-regulate the degranulation of mast cells. CD300A exists as multiple alternatively spliced isoforms and is subject to post-translational phosphorylation and glycosylation. The gene encoding CD300A maps to human chromosome 17, which comprises over 2.5% of the human genome and encodes over 1,200 genes.

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References:

1. The CMRF-35H gene structure predicts for an independently expressed member of an ITIM/ITAM pair of molecules localized to human chromosome 17.  |  Clark, GJ., et al. 2000. Tissue Antigens. 55: 101-9. PMID: 10746781
2. CMRF-35A, CMRF-35H: potential new CD.  |  Clark, GJ., et al. 2002. J Biol Regul Homeost Agents. 16: 233-5. PMID: 12456026
3. Molecular cloning of a novel member of the immunoglobulin gene superfamily homologous to the polymeric immunoglobulin receptor.  |  Jackson, DG., et al. 1992. Eur J Immunol. 22: 1157-63. PMID: 1349532
4. The inhibitory receptor IRp60 (CD300a) is expressed and functional on human mast cells.  |  Bachelet, I., et al. 2005. J Immunol. 175: 7989-95. PMID: 16339535
5. The CD300a (IRp60) inhibitory receptor is rapidly up-regulated on human neutrophils in response to inflammatory stimuli and modulates CD32a (FcgammaRIIa) mediated signaling.  |  Alvarez, Y., et al. 2008. Mol Immunol. 45: 253-8. PMID: 17588661
6. Suppression of normal and malignant kit signaling by a bispecific antibody linking kit with CD300a.  |  Bachelet, I., et al. 2008. J Immunol. 180: 6064-9. PMID: 18424727
7. CD300a/c regulate type I interferon and TNF-alpha secretion by human plasmacytoid dendritic cells stimulated with TLR7 and TLR9 ligands.  |  Ju, X., et al. 2008. Blood. 112: 1184-94. PMID: 18535206
8. The CMRF-35 mAb recognizes a second leukocyte membrane molecule with a domain similar to the poly Ig receptor.  |  Green, BJ., et al. 1998. Int Immunol. 10: 891-9. PMID: 9701027