CB1/Cannabinoid Receptor 1/CNR1抗体 (2F9) 推荐用于 mouse, rat 和 human 来源的CB1 WB, IP 和 ELISA检测

CB1/Cannabinoid Receptor 1/CNR1 抗体 (2F9): sc-293419

CB1/Cannabinoid Receptor 1/CNR1抗体 (2F9) is rated 4.0 out of 5 by 5.
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Datasheets

    • 抗-CB1/Cannabinoid Receptor 1/CNR1 抗体 2F9 是小鼠单克隆 IgG2b κ,CB1/Cannabinoid Receptor 1/CNR1抗体, 在8篇文献中引用,规格为100 µg/ml
    • raised against amino acids 1-110 representing recombinant partial length CB1 of human origin
    • 推荐用于 mouse, rat 和 human 来源的CB1 WB, IP 和 ELISA检测
    • 关于如何获取CB1/Cannabinoid Receptor 1/CNR1 (2F9): sc-293419免费10 µg小样,联系我们技术服务部门 (或者您当地的代理商)了解详情。
    • m-IgG Fc BP-HRP and m-IgG2b BP-HRP 是 CB1/Cannabinoid Receptor 1/CNR1 Antibody (2F9) for WB首选应用二抗。这些试剂现在以套装形式提供CB1/Cannabinoid Receptor 1/CNR1 Antibody (2F9) (见下方订购信息).
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Hello, I need to order CB1 and CB2 antibodies. Do you also provide positive and negative samples? I need the antibodies doing immunohistochemistry (IHC). Would the antibodies work for IHC?

Asked by: Farideh
Thank you for your question. We do not offer positive and negative tissue samples for IHC. This antibody is not currently recommended for IHC. We do offer an alternative, CB1 Antibody (C-11): sc-518035. This primary antibody is recommended and warrantied for IF (immunofluorescent staining of fresh or frozen tissues or cells fixed in methanol or acetone), however we are still gathering data to show on our website. For CB2, we offer the following, CB2 Antibody (3C7): sc-293188. Please note that neither one of these CB1 or CB2 antibodies has been tested yet on formalin-fixed paraffin-embedded tissues sections, and so IHC(P) is not yet a recommended application. Please check back for updates!
Answered by: Technical Support
Date published: 2019-02-02

For Western Blot, is it recommended to use denatured or non-denatured conditions with CB1 (2F9): sc-293419 antibody?

Asked by: Germaine
Thank you for your question. We recommend this antibody for use in denatured Western Blot conditions. It has not been validated for use in non-denatured conditions. Please contact our Technical Service Department for further details or inquiries.
Answered by: Technical Support
Date published: 2021-06-29
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Rated 4 out of 5 by from IHC and immunofluorescence for huan brain tissues IHC for human brain tissues, which were formalin-fixed, paraffin-embedded. Brown color is CB1R. Immunofluorescence for human brain tissues, which were formalin-fixed, paraffin-embedded. Red color is CB1R, green color is Iba1(microglia cell maker).
Date published: 2018-09-23
Rated 5 out of 5 by from much better than before work well in lysate from mouse brain cortex with a single specific band at expected mw ( detected additional band at 42 kD, not tested for possible upper bands),although we got not so strong signal considering the receptor brain expression
Date published: 2018-04-13
Rated 1 out of 5 by from Not working for Brain tissue ImmunoHistology Tried the Sample test for Fluorescence ImmunoHistology -Brain perfused with 4% PFA, transcardially -non-embedded free-floting section (40um) -"Antigene retrieval" at 80° in 10mM NatriumCitrate -PBS buffer -Concentration 1:100---Not tried for WB
Date published: 2018-01-04
Rated 5 out of 5 by from High specificity with dilutions around 1:400 This antibody works well in Western blot (1:400) for mouse brain in alcoholism model
Date published: 2017-12-25
Rated 5 out of 5 by from High specificity with dilutions around 1:200 This antibody works well in Western blot (1:200) and immunohistochemistry (1:100) for placental explants
Date published: 2017-05-09
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