Biotin抗体 (33) 推荐用于 both free and carrier protein bound biotin WB, IP 和 IF检测

Biotin 抗体 (33): sc-101339

Biotin抗体 (33) is rated 4.4 out of 5 by 5.
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Datasheets

    • Biotin抗体(33)是小鼠单克隆IgG1 (kappa轻链),规格为200 µg/ml
    • raised against Biotin
    • Biotin 抗体 (33) 推荐用于 WB, IP 和 IF,检测 both free and carrier protein bound biotin ; also recommended for detection of biocytin
    • 抗-Biotin 抗体 (33) 可偶联 琼脂糖 用于 IP; HRP 用于 WB, IHC(P) 和 ELISA; 还可偶联 藻红蛋白 或者 FITC 用于 IF, IHC(P) 和 FCM
    • 还可偶联Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594Alexa Fluor® 647,用于WB (RGB), IF, IHC(P) 和 FCM, 以及用于RGB荧光成像系统,例如iBright™ FL1000, FluorChem™, Typhoon, Azure和其他类似的系统
    • 还可偶联Alexa Fluor® 680Alexa Fluor® 790, 用于WB (NIR), IF 和 FCM; 以及用于近红外(NIR)检测系统,如LI-COR®/Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure和类似系统
    • 查看我们强烈推荐的传统抗小鼠IgG二抗的重组替代品 m-IgGκ BP-HRP (mouse IgGκ binding protein-HRP)
    • 关于如何获取Biotin (33): sc-101339免费10 µg小样,联系我们技术服务部门 (或者您当地的代理商)了解详情。

do you know the exact binding site on the Biotin molecule?

Asked by: Cäptn Val
Thank you for your question. The epitope region of Biotin Antibody (33): sc-101339 has not been determined yet, so the exact binding site of the antibody is not known.
Answered by: Tech Support Europe
Date published: 2019-09-05

What is the species reactivity of this Biotin monoclonal antibody? Can it be used with human samples?

Asked by: cjMara
Thank you for your inquiry. This antibody is not species specific but can be used detection of biotin in any sample, including human.
Answered by: Technical Service 11
Date published: 2017-01-19
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Rated 5 out of 5 by from works well Got a sample first, but then purchased the full one. Works well, and 1:1000 dilution is enough for western blot, although the datasheet says 1:10(!)
Date published: 2019-11-15
Rated 5 out of 5 by from Superb WB Very nice western blot data; strong signal with low background
Date published: 2017-02-03
Rated 5 out of 5 by from strong signal This antibody is an excellent choice to detecting free and carrier protein bound biotin and biocytin.
Date published: 2016-12-16
Rated 3 out of 5 by from Robust bands seen in WB using Biotin conjugated Robust bands seen in WB using Biotin conjugated goat IgG -SCBT QC
Date published: 2015-02-19
Rated 4 out of 5 by from ChIP publishable data with human HeLa ChIP publishable data with human HeLa - SCBT Publication Review
Date published: 2013-09-01
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