Bcr 抗体 (G6): sc-104

4.3(7)

- Bcr抗体(G6)是小鼠单克隆IgG₁ κ, 在18篇文献中引用，规格为200 µg/ml
- raised against a synthetic peptide from the amino acid sequence of the Bcr gene product
- 抗-Bcr 抗体 (G6) 推荐用于 WB, IP, IF 和 IHC(P)，检测mouse, rat 和human 来源的 Bcr/Abl p190 and Bcr/Abl p210 fusion proteins
- 抗-Bcr 抗体 (G6) 可偶联 琼脂糖 用于 IP; HRP 用于 WB, IHC(P) 和 ELISA; 还可偶联 藻红蛋白 或者 FITC 用于 IF, IHC(P) 和 FCM
- 还可偶联Alexa Fluor^® 488, Alexa Fluor^® 546, Alexa Fluor^® 594 和 Alexa Fluor^® 647，用于WB (RGB), IF, IHC(P) 和 FCM, 以及用于RGB荧光成像系统，例如iBright™ FL1000, FluorChem™, Typhoon, Azure和其他类似的系统
- 还可偶联Alexa Fluor^® 680 和 Alexa Fluor^® 790，用于WB (NIR), IF 和 FCM; 以及用于近红外（NIR）检测系统，如LI-COR^®/Odyssey^®, iBright™ FL1000, FluorChem™, Typhoon, Azure和类似系统
- 关于如何获取Bcr (G6): sc-104的免费10 µg小样，联系我们技术服务部门 (或者您当地的代理商)了解详情。
- m-IgG Fc BP-HRP and m-IgG₁ BP-HRP 是 Bcr Antibody (G6) for WB and IHC(P)首选应用二抗。这些试剂现在以套装形式提供Bcr Antibody (G6) (见下方订购信息).

References:

The BCR gene and philadelphia chromosome-positive leukemogenesis. | Laurent, E., et al. 2001. Cancer Res. 61: 2343-55. PMID: 11289094
Bcr encodes a GTPase-activating protein for p21rac. | Diekmann, D., et al. 1991. Nature. 351: 400-2. PMID: 1903516
Identification of two normal bcr gene products in the cytoplasm. | Dhut, S., et al. 1988. Oncogene. 3: 561-6. PMID: 3078961
The correlation of breakpoint cluster region rearrangement and p210 phl/abl expression with morphological analysis of Ph-negative chronic myeloid leukemia and other myeloproliferative diseases. | Wiedemann, LM., et al. 1988. Blood. 71: 349-55. PMID: 3276362
Evidence that the phl gene encodes a 160,000-dalton phosphoprotein with associated kinase activity. | Stam, K., et al. 1987. Mol Cell Biol. 7: 1955-60. PMID: 3299055
Evidence of a new chimeric bcr/c-abl mRNA in patients with chronic myelocytic leukemia and the Philadelphia chromosome. | Stam, K., et al. 1985. N Engl J Med. 313: 1429-33. PMID: 3864009
An alteration of the human c-abl protein in K562 leukemia cells unmasks associated tyrosine kinase activity. | Konopka, JB., et al. 1984. Cell. 37: 1035-42. PMID: 6204766
A cellular oncogene is translocated to the Philadelphia chromosome in chronic myelocytic leukaemia. | de Klein, A., et al. 1982. Nature. 300: 765-7. PMID: 6960256
Balanced parental contribution to the ABL component of the BCR-ABL gene in chronic myeloid leukemia. | Melo, JV., et al. 1995. Leukemia. 9: 734-9. PMID: 7723412

种属	基因名称	基因ID	染色质位置	亚型（mRNA）即位号码	蛋白质即位号码	OMIM™ 号码
Human	BCR	Entrez Gene ID: 613	22q11.23	NM_004327, NM_021574	UniProt ID: P11274	608232
Mouse	Bcr	Entrez Gene ID: 110279	10 B5.3	XM_006513060, XM_006513061, NM_001081412	UniProt ID: Q6PAJ1	N/A

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Protocols Online

针对哺乳动物和非哺乳动物蛋白靶点的单克隆抗体基本上代表了多克隆抗体覆盖的所有靶点。我们提供超过8300种ImmunoCruz^®偶联抗体，从而可以省去使用二抗。

圣克鲁斯生物技术的抗体有超过36万的研究引文。抗Bcr抗体(G6)在各种科学出版物中有18篇引文。

针对哺乳动物靶标蛋白的一抗，如抗Bcr 抗体 (G6)，推荐用于检测一系列哺乳动物物种，主要有小鼠，大鼠和人物种。许多哺乳动物抗体也非常适合兽医研究，与羊、猪、羊、猫、犬、牛和马的蛋白靶点反应。圣克鲁斯生物技术的单克隆一抗适用于实验室研究应用，不用于诊断或治疗。

Bcr 抗体 (G6) Specifications

替代产品名称

Bcr

产品克隆名称

分类

一抗

Clonality

单克隆抗体

抗体同型

IgG₁ kappa light chain

氨基酸

抗原表位靶标

Bcr

抗原表位种属

human

产品反应性/检测

Bcr/Abl p190 and Bcr/Abl p210 fusion proteins

种属反应性/检测

mouse, rat, human

其它哺乳动物反应性

N/A

其它非哺乳动物反应性

N/A

应用

WB, IP, IF, IHC(P)

瓶的信息

Each vial contains 200 µg IgG₁ kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

研究引文

18篇各种科学出版物的引文

可选的偶联物/形式

AC: Agarose AF488: Alexa Fluor^® 488 AF546: Alexa Fluor^® 546 AF594: Alexa Fluor^® 594 AF647: Alexa Fluor^® 647 AF680: Alexa Fluor^® 680 AF790: Alexa Fluor^® 790 FITC: Fluoroscein HRP: Horseradish Peroxidase PE: Phycoerythrin

针对非哺乳动物靶点的一抗在检测植物、病毒、细菌、鸟类、鱼类、酵母和无脊椎动物的蛋白质方面非常有用。例如果蝇,斑马鱼, 非洲爪蟾蜍, 拟南芥, 秀丽隐杆线虫, 梭菌, 弯曲杆菌, 幽门螺杆菌, 肝炎, HIV, HPV, HSV, 流行性感冒, 克雷白氏杆菌, 结核分支杆菌, 疟原虫, 假单胞菌, RSV, 酿酒酵母, 沙门氏菌, 血吸虫, 志贺氏杆菌 和 葡萄球菌.

圣克鲁斯生物技术单克隆抗体可用于流式细胞术、免疫沉淀、Western blot和免疫染色等分析。抗Bcr抗体(G6)推荐用于以下应用：WB (Western blotting), IP (Immunoprecipitation), IF (Immunofluorescence), IHC(P) (Immunohistochemistry - Paraffin Embedded).

我们还有小鼠二抗、对照血清和对照免疫球蛋白，可与我们的单克隆一抗联合使用。

组织提取物，核提取物和全细胞裂解物与圣克鲁斯生物技术单克隆抗体使用，作为western blotting或免疫沉淀阳性对照。核提取物是理想的western blot分析或凝胶移位分析阳性对照。磷酸化富集的全细胞裂解液和组织提取物可用于磷酸化特异性抗体阳性对照。

单克隆二抗有多种偶联形式，包括：Biotin (B), CruzFluor™ 790, 680, 647, 594, 555, and 488, FITC, HRP, PerCP, PerCP-Cy5.5, Texas Red (TR), 和TRITC (tetramethyl rhodamine isothiocyanate) (R)，用于研究应用，像流式细胞术（FCM），免疫荧光（IF），免疫组织化学（IHC）染色和蛋白印迹（WB）。不用于诊断或治疗。

偶联和非偶联的亲和纯化的免疫球蛋白(IgGs)，用于流式细胞术（FCM），免疫荧光（IF），免疫组织化学（IHC），免疫沉淀（IP）和蛋白印迹（WB）应用，作为阴性对照。

正常血清用于免疫组织化学（IHC）应用，作为封闭试剂。所使用的血清应与二抗的种属相匹配。

優選的 c-Abl 抗體是 c-Abl 抗體 (24-11): sc-23.

I am using Bcr (G6): sc-104 monoclonal antibody to stain paraffin embedded tissue. Do you recommend antigen retrieval when using this antibody?

Asked by: Dr Ninau Qelp

We recommend performing antigen retrieval with sodium citrate buffer (pH 6) and heat. The full protocol can be found here: https://www.scbt.com/scbt/resources/protocols/immunoperoxidase-staining

Answered by: Technical Support

Date published: 2017-02-24

Rated 4 out of 5 by LR from Worked well in WB with published results Worked well in WB with published results obtained from K562 cell samples. -SCBT Publication Review

Date published: 2015-05-31

Rated 5 out of 5 by LM from Good IF data with human HEK293 and BHK Good IF data with human HEK293 and BHK-21 cells. -SCBT Publication Review

Date published: 2015-04-25

Rated 4 out of 5 by SH from This antibody worked well for IP This antibody worked well for IP, published with human K-562, KU812 CML and BC-1 cells. -SCBT Publication Review

Date published: 2015-04-11

Rated 4 out of 5 by ET from WB: Worked well in human p185wt WB: Worked well in human p185wt, Ramos, K-562 and TF-1 treated and untreated. -SCBT Publication Review

Date published: 2015-03-26

Rated 5 out of 5 by JR from Publishable Western blot results using M2 Publishable Western blot results using M2 macrophages. -SCBT Publication Review

Date published: 2015-02-01

Rated 4 out of 5 by ET from Produced clear immunoperoxidase cytoplasmic Produced clear immunoperoxidase cytoplasmic staining in formalin fixed, paraffin-embedded human thyroid gland tissue. -SCBT QC

Date published: 2015-01-15

Rated 4 out of 5 by CJ from This antibody works well and can be used This antibody works well and can be used for IP with human HL60, KM cells. -SCBT Publication Review

Date published: 2015-01-13

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