BAAT 抗体 (ZA-18): sc-100475

BAAT Antibody (ZA-18) is an IgG₁ κ mouse monoclonal BAAT antibody (also designated BAAT antibody) that detects the BAAT protein of human origin by WB, IP and ELISA. BAAT Antibody (ZA-18) is available as the non-conjugated anti-BAAT antibody. BAAT (bile acid coenzyme A (CoA): amino acid N-acyltransferase), also known as BAT, BACAT or glycine N-choloyltransferase, is a member of the C/M/P thioester hydrolase family of proteins. Localizing to the cytoplasm and to peroxisomes, BAAT plays an essential role in bile acid metabolism, being the sole enzyme responsible for catalyzing the second step in the conjugation of bile acids to taurine or glycine. The first step in this reaction is the conversion of bile acids to CoA thioesters by ACSVL6 (bile acid CoA ligase). The conjugation of bile acids is important for its excretion into bile and it is also important for protection against toxicity by the accumulation of unconjugated bile acids. BAAT can be found in liver, pancreas, intestine and gallbladder mucosa. Mutations in the gene encoding BAAT have been associated with familial hypercholanemia (FHCA), a disease characterized by fat malabsorption, an increase in serum bile acid concentrations and itching.

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References:

1. Complex inheritance of familial hypercholanemia with associated mutations in TJP2 and BAAT.  |  Carlton, VE., et al. 2003. Nat Genet. 34: 91-6. PMID: 12704386
2. The human bile acid-CoA:amino acid N-acyltransferase functions in the conjugation of fatty acids to glycine.  |  O′Byrne, J., et al. 2003. J Biol Chem. 278: 34237-44. PMID: 12810727
3. Differential regulation of cytosolic and peroxisomal bile acid amidation by PPAR alpha activation favors the formation of unconjugated bile acids.  |  Solaas, K., et al. 2004. J Lipid Res. 45: 1051-60. PMID: 15026425
4. Molecular basis of intrahepatic cholestasis.  |  Carlton, VE., et al. 2004. Ann Med. 36: 606-17. PMID: 15768832
5. Bile acid coenzyme A: amino acid N-acyltransferase in the amino acid conjugation of bile acids.  |  Shonsey, EM., et al. 2005. Methods Enzymol. 400: 374-94. PMID: 16399361
6. Human and rat bile acid-CoA:amino acid N-acyltransferase are liver-specific peroxisomal enzymes: implications for intracellular bile salt transport.  |  Pellicoro, A., et al. 2007. Hepatology. 45: 340-8. PMID: 17256745
7. Quantification and regulation of the subcellular distribution of bile acid coenzyme A:amino acid N-acyltransferase activity in rat liver.  |  Styles, NA., et al. 2007. J Lipid Res. 48: 1305-15. PMID: 17379925
8. Genetic polymorphism of bile acid CoA: amino acid N-acyltransferase in Japanese individuals.  |  Tougou, K., et al. 2007. Drug Metab Pharmacokinet. 22: 125-8. PMID: 17495420
9. Inactivation of human liver bile acid CoA:amino acid N-acyltransferase by the electrophilic lipid, 4-hydroxynonenal.  |  Shonsey, EM., et al. 2008. J Lipid Res. 49: 282-94. PMID: 17965457