B-ATF 抗体 (WW8): sc-100974

5.0(3)

- B-ATF 抗体 WW8 是小鼠单克隆 IgG₁ κ，B-ATF抗体, 在17篇文献中引用，规格为100 µg/ml
- raised against a partial recombinant protein mapping within amino acids 34-126 of B-ATF of human origin
- 推荐用于 mouse, rat 和 human 来源的B-ATF WB, IP 和 ELISA检测
- 关于如何获取B-ATF (WW8): sc-100974的免费10 µg小样，联系我们技术服务部门 (或者您当地的代理商)了解详情。
- m-IgG Fc BP-HRP and m-IgG₁ BP-HRP 是 B-ATF Antibody (WW8) for WB首选应用二抗。这些试剂现在以套装形式提供B-ATF Antibody (WW8) (见下方订购信息).

References:

B-ATF functions as a negative regulator of AP-1 mediated transcription and blocks cellular transformation by Ras and Fos. | Echlin, DR., et al. 2000. Oncogene. 19: 1752-63. PMID: 10777209
B-ATF: a novel human bZIP protein that associates with members of the AP-1 transcription factor family. | Dorsey, MJ., et al. 1995. Oncogene. 11: 2255-65. PMID: 8570175
SFA-2, a novel bZIP transcription factor induced by human T-cell leukemia virus type I, is highly expressed in mature lymphocytes. | Hasegawa, H., et al. 1996. Biochem Biophys Res Commun. 222: 164-70. PMID: 8630063
IFP 35 forms complexes with B-ATF, a member of the AP1 family of transcription factors. | Wang, X., et al. 1996. Biochem Biophys Res Commun. 229: 316-22. PMID: 8954125
Genomic organization of human B-ATF, a target for regulation by EBV and HTLV-1. | Meyer, NP., et al. 1998. Mamm Genome. 9: 849-52. PMID: 9745044

种属	基因名称	基因ID	染色质位置	亚型（mRNA）即位号码	蛋白质即位号码	OMIM™ 号码
Human	BATF	Entrez Gene ID: 10538	14q24.3	NM_006399	UniProt ID: Q16520	n/a
Mouse	Batf	Entrez Gene ID: 53314	12 D2	NM_016767	UniProt ID: O35284	N/A

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Protocols Online

针对哺乳动物和非哺乳动物蛋白靶点的单克隆抗体基本上代表了多克隆抗体覆盖的所有靶点。我们提供超过8300种ImmunoCruz^®偶联抗体，从而可以省去使用二抗。

圣克鲁斯生物技术的抗体有超过36万的研究引文。抗B-ATF抗体(WW8)在各种科学出版物中有17篇引文。

针对哺乳动物靶标蛋白的一抗，如抗B-ATF 抗体 (WW8)，推荐用于检测一系列哺乳动物物种，主要有小鼠，大鼠和人物种。许多哺乳动物抗体也非常适合兽医研究，与羊、猪、羊、猫、犬、牛和马的蛋白靶点反应。圣克鲁斯生物技术的单克隆一抗适用于实验室研究应用，不用于诊断或治疗。

B-ATF 抗体 (WW8) Specifications

替代产品名称

BATF

产品克隆名称

WW8

分类

一抗

Clonality

单克隆抗体

抗体同型

IgG₁ kappa light chain

氨基酸

34-126

抗原表位靶标

B-ATF

抗原表位种属

human

产品反应性/检测

B-ATF

种属反应性/检测

mouse, rat, human

其它哺乳动物反应性

N/A

其它非哺乳动物反应性

N/A

应用

WB, IP, ELISA

瓶的信息

Each vial contains 100 µg IgG₁ kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

研究引文

17篇各种科学出版物的引文

可选的偶联物/形式

N/A

针对非哺乳动物靶点的一抗在检测植物、病毒、细菌、鸟类、鱼类、酵母和无脊椎动物的蛋白质方面非常有用。例如果蝇,斑马鱼, 非洲爪蟾蜍, 拟南芥, 秀丽隐杆线虫, 梭菌, 弯曲杆菌, 幽门螺杆菌, 肝炎, HIV, HPV, HSV, 流行性感冒, 克雷白氏杆菌, 结核分支杆菌, 疟原虫, 假单胞菌, RSV, 酿酒酵母, 沙门氏菌, 血吸虫, 志贺氏杆菌 和 葡萄球菌.

圣克鲁斯生物技术单克隆抗体可用于流式细胞术、免疫沉淀、Western blot和免疫染色等分析。抗B-ATF抗体(WW8)推荐用于以下应用：WB (Western blotting), IP (Immunoprecipitation), ELISA (Enzyme-Linked Immunosorbent Assay).

我们还有小鼠二抗、对照血清和对照免疫球蛋白，可与我们的单克隆一抗联合使用。

组织提取物，核提取物和全细胞裂解物与圣克鲁斯生物技术单克隆抗体使用，作为western blotting或免疫沉淀阳性对照。核提取物是理想的western blot分析或凝胶移位分析阳性对照。磷酸化富集的全细胞裂解液和组织提取物可用于磷酸化特异性抗体阳性对照。

单克隆二抗有多种偶联形式，包括：Biotin (B), CruzFluor™ 790, 680, 647, 594, 555, and 488, FITC, HRP, PerCP, PerCP-Cy5.5, Texas Red (TR), 和TRITC (tetramethyl rhodamine isothiocyanate) (R)，用于研究应用，像流式细胞术（FCM），免疫荧光（IF），免疫组织化学（IHC）染色和蛋白印迹（WB）。不用于诊断或治疗。

偶联和非偶联的亲和纯化的免疫球蛋白(IgGs)，用于流式细胞术（FCM），免疫荧光（IF），免疫组织化学（IHC），免疫沉淀（IP）和蛋白印迹（WB）应用，作为阴性对照。

正常血清用于免疫组织化学（IHC）应用，作为封闭试剂。所使用的血清应与二抗的种属相匹配。

Can you recommend a positive control to use with B-ATF (WW8): sc-100974 monoclonal antibody for Western blot?

Asked by: jenni

We recommend SW480 (sc-2219), HeLa (sc-2200), Jurkat (sc-2204), RAW 264.7 (sc-2212), NIH/3T3 (sc-2257) or PC-12 (sc-2250) whole cell lysates as we have great Western blot data showing specific detection of B-ATF in these cell lines.

Answered by: Technical Support 2

Date published: 2017-01-12

I am using the LiCor/Odyssey system. Which secondary antibody do you recommend using with B-ATF (WW8): sc-100974?

Asked by: jenni

We recommend using one of our our exclusive Mouse IgG Binding Proteins (m-IgGκ) conjugated to CFL 680 or CFL 790. They are suitable for use with Near-Infrared (NIR) detection systems, such as LI-COR/Odyssey and other comparable systems. A complete list of available binding proteins is available on our website here: https://www.scbt.com/scbt/browse/support-products-mouse-igg-binding-proteins/_/N-ecrety

Answered by: Technical Support 2

Date published: 2017-01-12

Rated 5 out of 5 by Bill28 from Great western data Outstanding western blot data; strong signal with low background

Date published: 2017-01-30

Rated 5 out of 5 by Sweetums from Good B-ATF detection Antibody produces reliable signal in Western blot for B-ATF and is recommended for mouse, rat, and human samples.

Date published: 2017-01-24

Rated 5 out of 5 by JM from WB and ChIP publishable data with human and WB and ChIP publishable data with human and mouse T-cells - SCBT Publication Review

Date published: 2014-09-20

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