ArgRS 抗体 (ZB-12): sc-100990

ArgRS Antibody (ZB-12) is an IgM mouse monoclonal ArgRS antibody (also designated ArgRS antibody) that detects the ArgRS protein of human origin by WB, IP and ELISA. ArgRS Antibody (ZB-12) is available as the non-conjugated anti-ArgRS antibody. The fidelity of protein synthesis requires efficient discrimination of amino acid substrates by aminoacyl-tRNA synthetases. ArgRS (Arginyl-tRNA synthetase), also known as RARS or DALRD1, belongs to the class-I aminoacyl-tRNA synthetase family that includes the related proteins, LeuRS, ValRS and IleRS. These proteins are large monomeric proteins and play a major role in catalyzing the aminoacylation of tRNA by their cognate amino acid. ArgRS localizes to the cytoplasm and exists as a monomer but can also associate with other tRNA synthetases and auxiliary proteins to form a multisubunit complex. In the presence of ATP, arginine (Arg) and tRNA, ArgRS joins Arg to tRNA(Arg) at its synthetic active site. Two cytoplasmic forms of ArgRS have been described in mammals, differing by the addition of a 73 amino acid sequence that is required for ArgRS assembly into the multisubunit complex.

Alexa Fluor^® is a trademark of Molecular Probes Inc., OR., USA

LI-COR^® and Odyssey^® are registered trademarks of LI-COR Biosciences

References:

1. Macromolecular assemblage of aminoacyl-tRNA synthetases: quantitative analysis of protein-protein interactions and mechanism of complex assembly.  |  Robinson, JC., et al. 2000. J Mol Biol. 304: 983-94. PMID: 11124041
2. Arginyl-tRNA synthetase with signature sequence KMSK from Bacillus stearothermophilus.  |  Li, J., et al. 2003. Biochem J. 376: 773-9. PMID: 13678419
3. Escherichia coli tRNA(4)(Arg)(UCU) induces a constrained conformation of the crucial Omega-loop of arginyl-tRNA synthetase.  |  Yao, YN., et al. 2004. Biochem Biophys Res Commun. 313: 129-34. PMID: 14672708
4. The tRNA-interacting factor p43 associates with mammalian arginyl-tRNA synthetase but does not modify its tRNA aminoacylation properties.  |  Guigou, L., et al. 2004. Biochemistry. 43: 4592-600. PMID: 15078106
5. Determinants in tRNA for activation of arginyl-tRNA synthetase: evidence that tRNA flexibility is required for the induced-fit mechanism.  |  Guigou, L. and Mirande, M. 2005. Biochemistry. 44: 16540-8. PMID: 16342945
6. Two forms of human cytoplasmic arginyl-tRNA synthetase produced from two translation initiations by a single mRNA.  |  Zheng, YG., et al. 2006. Biochemistry. 45: 1338-44. PMID: 16430231
7. Cloning and characterization of cDNA encoding a human arginyl-tRNA synthetase.  |  Girjes, AA., et al. 1995. Gene. 164: 347-50. PMID: 7590355
8. Cloning and analysis of a cDNA encoding mammalian arginyl-tRNA synthetase, a component of the multisynthetase complex with a hydrophobic N-terminal extension.  |  Lazard, M. and Mirande, M. 1993. Gene. 132: 237-45. PMID: 8224869
9. Macromolecular assemblage of aminoacyl-tRNA synthetases: identification of protein-protein interactions and characterization of a core protein.  |  Quevillon, S., et al. 1999. J Mol Biol. 285: 183-95. PMID: 9878398