AMPKβ1 抗体 (Z14): sc-100357

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Datasheets

- AMPK beta 1 抗体 Z14 是小鼠单克隆 IgG_2a κ，AMPKβ1抗体, 在4篇文献中引用，规格为100 µg/ml
- 免疫human物种的重组AMPKβ1
- 推荐用于 mouse, rat 和 human 来源的AMPKβ1 WB, IP, IF 和 ELISA检测
- 关于如何获取AMPKβ1 (Z14): sc-100357的免费10 µg小样，联系我们技术服务部门 (或者您当地的代理商)了解详情。
- m-IgG_2a BP-HRP 是AMPKβ1 Antibody (Z14) for WB首选应用二抗. 这些试剂现在以套装形式提供AMPKβ1 Antibody (Z14) (见下方订购信息).

SEE ALSO...

此外 AMPK 抗体包括 AMPK α 1, AMPK α 2, AMPK β1, AMPK β2 和 AMPK γ 2
ChemCruz™ Chemical AMPK Inhibitors or AMPK Activators可用于AMPK细胞反应的功能性分析

AMPKβ1 Antibody (Z14) - Western Blotting - Image 368192

AMPKβ1 Antibody (Z14) - Immunofluorescence - Image 35537

AMPKβ1 Antibody (Z14) - Western Blotting - Image 368193

AMPKβ1 Antibody (Z14) - Western Blotting - Image 33733

AMPKβ1 Antibody (Z14) - Western Blotting - Image 129061

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AMPKβ1 Antibody (Z14) is an IgG_2a κ mouse monoclonal AMPK beta 1 antibody (also designated AMPKβ1 antibody) that detects the AMPK beta 1 protein of mouse, rat and human origin by WB, IP, IF and ELISA. AMPKβ1 Antibody (Z14) is available as the non-conjugated anti-AMPK beta 1 antibody. AMPK (for 5′-AMP-activated protein kinase) is a heterotrimeric complex com-prising a catalytic α subunit and regulatory β and γ subunits. It protects cells from stresses that cause ATP depletion by switching off ATP-consuming bio-synthetic pathways. AMPK is activated by high AMP and low ATP through a mechanism involving allosteric regulation, promotion of phosphorylation by an upstream protein kinase known as AMPK kinase, and inhibition of dephosphorylation. Activated AMPK can phosphorylate and regulate in vivo hydroxy-methylglutaryl-CoA reductase and acetyl-CoA carboxylase, which are key regulatory enzymes of sterol synthesis and fatty acid synthesis, respectively. The human AMPKα1 and AMPKα2 genes encode 548 amino acid and 552 amino acid proteins, respectively. Human AMPKβ1 encodes a 271 amino acid protein and human AMPKβ2 encodes a 272 amino acid protein. The human AMPKγ1 gene encodes a 331 amino acid protein. Human AMPKγ2 and AMPKγ3, which are 569 and 492 amino acid proteins, respectively, contain unique N-terminal domains and may participate directly in the binding of AMP within the AMPK complex.

For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.

Alexa Fluor^® is a trademark of Molecular Probes Inc., OR., USA

LI-COR^® and Odyssey^® are registered trademarks of LI-COR Biosciences

References:

Characterization of AMP-activated protein kinase gamma-subunit isoforms and their role in AMP binding. | Cheung, PC., et al. 2000. Biochem J. 346 Pt 3: 659-69. PMID: 10698692
Mammalian AMP-activated protein kinase subfamily. | Stapleton, D., et al. 1996. J Biol Chem. 271: 611-4. PMID: 8557660
The AMP-activated protein kinase--fuel gauge of the mammalian cell? | Hardie, DG. and Carling, D. 1997. Eur J Biochem. 246: 259-73. PMID: 9208914
AMP-activated protein kinase isoenzyme family: subunit structure and chromosomal location. | Stapleton, D., et al. 1997. FEBS Lett. 409: 452-6. PMID: 9224708
Identification of a novel AMP-activated protein kinase beta subunit isoform that is highly expressed in skeletal muscle. | Thornton, C., et al. 1998. J Biol Chem. 273: 12443-50. PMID: 9575201

种属	基因名称	基因ID	染色质位置	亚型（mRNA）即位号码	蛋白质即位号码	OMIM™ 号码
Human	PRKAB1	Entrez Gene ID: 5564	12q24.23	XM_005253909, NM_006253	UniProt ID: Q9Y478	602740
Mouse	Prkab1	Entrez Gene ID: 19079	5 F	XM_006530201, NM_031869	UniProt ID: Q9R078	N/A

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Protocols Online

针对哺乳动物和非哺乳动物蛋白靶点的单克隆抗体基本上代表了多克隆抗体覆盖的所有靶点。我们提供超过8300种ImmunoCruz^®偶联抗体，从而可以省去使用二抗。

圣克鲁斯生物技术的抗体有超过36万的研究引文。抗AMPKβ1抗体(Z14)在各种科学出版物中有4篇引文。

针对哺乳动物靶标蛋白的一抗，如抗AMPKβ1 抗体 (Z14)，推荐用于检测一系列哺乳动物物种，主要有小鼠，大鼠和人物种。许多哺乳动物抗体也非常适合兽医研究，与羊、猪、羊、猫、犬、牛和马的蛋白靶点反应。圣克鲁斯生物技术的单克隆一抗适用于实验室研究应用，不用于诊断或治疗。

AMPKβ1 抗体 (Z14) Specifications

替代产品名称

AMPKβ1

产品克隆名称

Z14

分类

一抗

Clonality

单克隆抗体

抗体同型

IgG_2a kappa light chain

氨基酸

1-271

抗原表位靶标

AMPKβ1

抗原表位种属

human

产品反应性/检测

AMPKβ1

种属反应性/检测

mouse, rat, human

其它哺乳动物反应性

N/A

其它非哺乳动物反应性

N/A

应用

WB, IP, IF, ELISA

瓶的信息

Each vial contains 100 µg IgG_2a kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

研究引文

4篇各种科学出版物的引文

可选的偶联物/形式

N/A

针对非哺乳动物靶点的一抗在检测植物、病毒、细菌、鸟类、鱼类、酵母和无脊椎动物的蛋白质方面非常有用。例如果蝇,斑马鱼, 非洲爪蟾蜍, 拟南芥, 秀丽隐杆线虫, 梭菌, 弯曲杆菌, 幽门螺杆菌, 肝炎, HIV, HPV, HSV, 流行性感冒, 克雷白氏杆菌, 结核分支杆菌, 疟原虫, 假单胞菌, RSV, 酿酒酵母, 沙门氏菌, 血吸虫, 志贺氏杆菌 和 葡萄球菌.

圣克鲁斯生物技术单克隆抗体可用于流式细胞术、免疫沉淀、Western blot和免疫染色等分析。抗AMPKβ1抗体(Z14)推荐用于以下应用：WB (Western blotting), IP (Immunoprecipitation), IF (Immunofluorescence), ELISA (Enzyme-Linked Immunosorbent Assay).

我们还有小鼠二抗、对照血清和对照免疫球蛋白，可与我们的单克隆一抗联合使用。

组织提取物，核提取物和全细胞裂解物与圣克鲁斯生物技术单克隆抗体使用，作为western blotting或免疫沉淀阳性对照。核提取物是理想的western blot分析或凝胶移位分析阳性对照。磷酸化富集的全细胞裂解液和组织提取物可用于磷酸化特异性抗体阳性对照。

单克隆二抗有多种偶联形式，包括：Biotin (B), CruzFluor™ 790, 680, 647, 594, 555, and 488, FITC, HRP, PerCP, PerCP-Cy5.5, Texas Red (TR), 和TRITC (tetramethyl rhodamine isothiocyanate) (R)，用于研究应用，像流式细胞术（FCM），免疫荧光（IF），免疫组织化学（IHC）染色和蛋白印迹（WB）。不用于诊断或治疗。

偶联和非偶联的亲和纯化的免疫球蛋白(IgGs)，用于流式细胞术（FCM），免疫荧光（IF），免疫组织化学（IHC），免疫沉淀（IP）和蛋白印迹（WB）应用，作为阴性对照。

正常血清用于免疫组织化学（IHC）应用，作为封闭试剂。所使用的血清应与二抗的种属相匹配。

I am using AMPKβ1 (Z14): sc-100357 for immunofluorescence, what dilution of this antibody should I use?

Asked by: Cweed

Thank you for your question. We recommend a starting dilution of 1:50. However, optimal antibody concentration should be determined by titration. Please find our full immunofluorescence protocol here: https://www.scbt.com/scbt/resources/protocols/immunofluorescence-cell-staining

Answered by: Technical Support

Date published: 2017-03-24

Rated 5 out of 5 by SigmaHU from Good Antibody for WB We can get clear band. Application: WB Sample: rat microglia culture

Date published: 2020-08-11

Rated 5 out of 5 by Yuanhong from good product This antibody works pretty good for WB experiments.

Date published: 2018-09-11

Rated 4 out of 5 by CB75005 from WB AMPK β1 (Z-14) One unique strong and clear band at 38 kDa. 40 ug of whole cell extract from 12 month old murine hepatocellular carcinoma (2) tumoral tissue (1) adjacent non tumoral tissue. Dilution 1/1000.

Date published: 2017-10-13

Rated 5 out of 5 by Jinhua Meng from Great features I applied a sample one month ago and I did Westen Blotting (dilution 1:100) at white adipose tissue of mouse, the results is excellent.

Date published: 2017-09-20

Rated 5 out of 5 by FL from Strong band in WB in HeLa whole cell lysate Strong band in WB in HeLa whole cell lysate. -SCBT QC

Date published: 2015-05-03

Rated 5 out of 5 by JT from Clear band in WB in lambda protein phosphatase Clear band in WB in lambda protein phosphatase treated differentiated C2C12 whole cell lysate. -SCBT QC

Date published: 2015-04-25

Rated 5 out of 5 by ET from Produced excellent immunofluorescence nuclear Produced excellent immunofluorescence nuclear and cytoplasmic staining in paraformaldehyde-fixed HeLa cells. -SCBT QC

Date published: 2015-01-25

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AMPKβ1 抗体 (Z14): sc-100357

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References:

AMPKβ1 抗体 (Z14) Specifications

替代产品名称

产品克隆名称

分类

Clonality

抗体同型

氨基酸

抗原表位靶标

抗原表位种属

产品反应性/检测

种属反应性/检测

其它哺乳动物反应性

其它非哺乳动物反应性

应用

瓶的信息

研究引文

推荐的二抗检测试剂

可选的偶联物/形式

I am using AMPKβ1 (Z14): sc-100357 for immunofluorescence, what dilution of this antibody should I use?

AMPKβ1 抗体 (Z14): sc-100357

QUICK LINKS

SEE ALSO...

References:

AMPKβ1 抗体 (Z14) Specifications

替代产品名称

产品克隆名称

分类

Clonality

抗体同型

氨基酸

抗原表位靶标

抗原表位种属

产品反应性/检测

种属反应性/检测

其它哺乳动物反应性

其它非哺乳动物反应性

应用

瓶的信息

研究引文

推荐的二抗检测试剂

可选的偶联物/形式

I am using AMPK&beta;1 (Z14): sc-100357 for immunofluorescence, what dilution of this antibody should I use?

I am using AMPKβ1 (Z14): sc-100357 for immunofluorescence, what dilution of this antibody should I use?