AMPK beta 1抗体 (Z14) 推荐用于 mouse, rat 和 human 来源的AMPKβ1 WB, IP, IF 和 ELISA检测

AMPKβ1 抗体 (Z14): sc-100357

AMPKβ1抗体 (Z14) is rated 4.8 out of 5 by 6.
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Datasheets

    • 抗-AMPK beta 1 抗体 Z14 是小鼠单克隆 IgG2a (kappa轻链),AMPKβ1抗体,规格为100 µg/ml
    • 免疫human物种的重组AMPKβ1
    • 推荐用于 mouse, rat 和 human 来源的AMPKβ1 WB, IP, IF 和 ELISA检测
    • 查看我们强烈推荐的传统抗小鼠IgG二抗的重组替代品 m-IgGκ BP-HRP (mouse IgGκ binding protein-HRP)
    • 关于如何获取AMPKβ1 (Z14): sc-100357免费10 µg小样,联系我们技术服务部门 (或者您当地的代理商)了解详情。
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I am using AMPKβ1 (Z14): sc-100357 for immunofluorescence, what dilution of this antibody should I use?

Asked by: Cweed
Thank you for your question. We recommend a starting dilution of 1:50. However, optimal antibody concentration should be determined by titration. Please find our full immunofluorescence protocol here: https://www.scbt.com/scbt/resources/protocols/immunofluorescence-cell-staining
Answered by: Technical Support
Date published: 2017-03-24
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Rated 5 out of 5 by from good product This antibody works pretty good for WB experiments.
Date published: 2018-09-11
Rated 4 out of 5 by from WB AMPK β1 (Z-14) One unique strong and clear band at 38 kDa. 40 ug of whole cell extract from 12 month old murine hepatocellular carcinoma (2) tumoral tissue (1) adjacent non tumoral tissue. Dilution 1/1000.
Date published: 2017-10-13
Rated 5 out of 5 by from Great features I applied a sample one month ago and I did Westen Blotting (dilution 1:100) at white adipose tissue of mouse, the results is excellent.
Date published: 2017-09-20
Rated 5 out of 5 by from Strong band in WB in HeLa whole cell lysate Strong band in WB in HeLa whole cell lysate. -SCBT QC
Date published: 2015-05-03
Rated 5 out of 5 by from Clear band in WB in lambda protein phosphatase Clear band in WB in lambda protein phosphatase treated differentiated C2C12 whole cell lysate. -SCBT QC
Date published: 2015-04-25
Rated 5 out of 5 by from Produced excellent immunofluorescence nuclear Produced excellent immunofluorescence nuclear and cytoplasmic staining in paraformaldehyde-fixed HeLa cells. -SCBT QC
Date published: 2015-01-25
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