α_1A-AR 抗体 (4D8): sc-100291

4.7(3)

- alpha 1A-AR 抗体 4D8 是小鼠单克隆 IgG₁ κ，α_1A-AR抗体, 在12篇文献中引用，规格为100 µg/ml
- raised against an N-terminal region of α_1A-AR of human origin
- 推荐用于 mouse, rat 和 human 来源的α_1A-AR WB, IP 和 ELISA检测
- 关于如何获取α_1A-AR (4D8): sc-100291的免费10 µg小样，联系我们技术服务部门 (或者您当地的代理商)了解详情。
- 目前，我们还没有完成α_1A-AR 抗体 (4D8)的首选二抗检测试剂的鉴定。这项工作正在进行中。

References:

alpha 1-Adrenergic receptor subtypes differentially control the cell cycle of transfected CHO cells through a cAMP-dependent mechanism involving p27Kip1. | Shibata, K., et al. 2003. J Biol Chem. 278: 672-8. PMID: 12409310
Differential regulation of the cell cycle by alpha1-adrenergic receptor subtypes. | Gonzalez-Cabrera, PJ., et al. 2004. Endocrinology. 145: 5157-67. PMID: 15297446
Beta-arrestin-dependent spontaneous alpha1a-adrenoceptor endocytosis causes intracellular transportation of alpha-blockers via recycling compartments. | Pediani, JD., et al. 2005. Mol Pharmacol. 67: 992-1004. PMID: 15626751
Mammalian tolloid alters subcellular localization, internalization, and signaling of alpha(1a)-adrenergic receptors. | Xu, Q., et al. 2006. Mol Pharmacol. 70: 532-41. PMID: 16690783
Evidence of alpha1-adrenoceptor functional changes in omental arteries of patients with end-stage renal disease. | Cruz-Domínguez, MP., et al. 2008. Auton Autacoid Pharmacol. 28: 19-27. PMID: 18257748
Cloning, functional expression and tissue distribution of human cDNA for the alpha 1C-adrenergic receptor. | Hirasawa, A., et al. 1993. Biochem Biophys Res Commun. 195: 902-9. PMID: 8396931
Molecular cloning, genomic characterization and expression of novel human alpha1A-adrenoceptor isoforms. | Chang, DJ., et al. 1998. FEBS Lett. 422: 279-83. PMID: 9490024
Examination of alpha 1 adrenoceptor subtypes in the human ureter. | Itoh, Y., et al. 2007. Int J Urol. 14: 749-53. PMID: 17681068

种属	基因名称	基因ID	染色质位置	亚型（mRNA）即位号码	蛋白质即位号码	OMIM™ 号码
Human	ADRA1A	Entrez Gene ID: 148	8p21.2	XM_006716292, XM_006716293, XM_011544411, XM_011544412, XM_017013094, XM_017013095, XM_017013096, NM_000680, NM_001322502, NM_001322503, NM_001322504, NM_033302, NM_033303, NM_033304	UniProt ID: P35348	104221

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针对哺乳动物和非哺乳动物蛋白靶点的单克隆抗体基本上代表了多克隆抗体覆盖的所有靶点。我们提供超过8300种ImmunoCruz^®偶联抗体，从而可以省去使用二抗。

圣克鲁斯生物技术的抗体有超过36万的研究引文。抗α_1A-AR抗体(4D8)在各种科学出版物中有12篇引文。

针对哺乳动物靶标蛋白的一抗，如抗α_1A-AR 抗体 (4D8)，推荐用于检测一系列哺乳动物物种，主要有小鼠，大鼠和人物种。许多哺乳动物抗体也非常适合兽医研究，与羊、猪、羊、猫、犬、牛和马的蛋白靶点反应。圣克鲁斯生物技术的单克隆一抗适用于实验室研究应用，不用于诊断或治疗。

α_1A-AR 抗体 (4D8) Specifications

替代产品名称

ADRA1A

产品克隆名称

4D8

分类

一抗

Clonality

单克隆抗体

抗体同型

IgG₁ kappa light chain

氨基酸

1-28

抗原表位靶标

α_1A-AR

抗原表位种属

human

产品反应性/检测

α_1A-AR

种属反应性/检测

mouse, rat, human

其它哺乳动物反应性

N/A

其它非哺乳动物反应性

N/A

应用

WB, IP, ELISA

瓶的信息

Each vial contains 100 µg IgG₁ kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

研究引文

12篇各种科学出版物的引文

可选的偶联物/形式

N/A

针对非哺乳动物靶点的一抗在检测植物、病毒、细菌、鸟类、鱼类、酵母和无脊椎动物的蛋白质方面非常有用。例如果蝇,斑马鱼, 非洲爪蟾蜍, 拟南芥, 秀丽隐杆线虫, 梭菌, 弯曲杆菌, 幽门螺杆菌, 肝炎, HIV, HPV, HSV, 流行性感冒, 克雷白氏杆菌, 结核分支杆菌, 疟原虫, 假单胞菌, RSV, 酿酒酵母, 沙门氏菌, 血吸虫, 志贺氏杆菌 和 葡萄球菌.

圣克鲁斯生物技术单克隆抗体可用于流式细胞术、免疫沉淀、Western blot和免疫染色等分析。抗α_1A-AR抗体(4D8)推荐用于以下应用：WB (Western blotting), IP (Immunoprecipitation), ELISA (Enzyme-Linked Immunosorbent Assay).

我们还有小鼠二抗、对照血清和对照免疫球蛋白，可与我们的单克隆一抗联合使用。

组织提取物，核提取物和全细胞裂解物与圣克鲁斯生物技术单克隆抗体使用，作为western blotting或免疫沉淀阳性对照。核提取物是理想的western blot分析或凝胶移位分析阳性对照。磷酸化富集的全细胞裂解液和组织提取物可用于磷酸化特异性抗体阳性对照。

单克隆二抗有多种偶联形式，包括：Biotin (B), CruzFluor™ 790, 680, 647, 594, 555, and 488, FITC, HRP, PerCP, PerCP-Cy5.5, Texas Red (TR), 和TRITC (tetramethyl rhodamine isothiocyanate) (R)，用于研究应用，像流式细胞术（FCM），免疫荧光（IF），免疫组织化学（IHC）染色和蛋白印迹（WB）。不用于诊断或治疗。

偶联和非偶联的亲和纯化的免疫球蛋白(IgGs)，用于流式细胞术（FCM），免疫荧光（IF），免疫组织化学（IHC），免疫沉淀（IP）和蛋白印迹（WB）应用，作为阴性对照。

正常血清用于免疫组织化学（IHC）应用，作为封闭试剂。所使用的血清应与二抗的种属相匹配。

How selective is this antibody for alpha-1A? We need to address the selectivity issue in light of Michel et al. (2009) "How reliable are G-protein-coupled receptor antibodies?" NAUNYN-SCHMIEDEBERGS ARCHIVES OF PHARMACOLOGY 379: 386-388.

Asked by: cpoon

Thank you for your question. The manuscript you mention recommends validating by IHC, but I would like to aslso suggest that also using siRNAs and CRISPR followed by a WB would also provide validation results to determine the reliability of the antibody. You can find recommended gene silencing products on the antibody product block: https://www.scbt.com/scbt/product/alpha1a-ar-antibody-4d8?requestFrom=search If you have any further questions, please contact Technical Service by phone, (800)-457-3801 option 2, email <[email protected]>, or by live chat directly on our website, www.scbt.com.

Answered by: Tech Service

Date published: 2017-06-28

For Western Blot, is it recommended to use denatured or non-denatured conditions with α1A-AR (4D8): sc-100291 antibody?

Asked by: Germaine

Thank you for your question. We recommend this antibody for use in denatured Western Blot conditions. It has not been validated for use in non-denatured conditions. Please contact our Technical Service Department for further details or inquiries.

Answered by: Technical Support

Date published: 2017-03-27

Rated 5 out of 5 by LindaS from Worked very well I used this antibody on mice tissues (liver, muscle, aorta) and it worked very well.

Date published: 2018-04-06

Rated 5 out of 5 by Xiao Qi from positive results Product sc-100291 is used for kinase assay using human myeloid cells,positive results!

Date published: 2016-12-17

Rated 4 out of 5 by MS from Distinct band observed by WB using HL Distinct band observed by WB using HL-60 whole cell lysate. -SCBT QC

Date published: 2014-02-27

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α_1A-AR 抗体 (4D8): sc-100291

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References:

α_1A-AR 抗体 (4D8) Specifications

替代产品名称

产品克隆名称

分类

Clonality

抗体同型

氨基酸

抗原表位靶标

抗原表位种属

产品反应性/检测

种属反应性/检测

其它哺乳动物反应性

其它非哺乳动物反应性

应用

瓶的信息

研究引文

推荐的二抗检测试剂

可选的偶联物/形式

How selective is this antibody for alpha-1A? We need to address the selectivity issue in light of Michel et al. (2009) "How reliable are G-protein-coupled receptor antibodies?" NAUNYN-SCHMIEDEBERGS ARCHIVES OF PHARMACOLOGY 379: 386-388.

For Western Blot, is it recommended to use denatured or non-denatured conditions with α1A-AR (4D8): sc-100291 antibody?

α1A-AR 抗体 (4D8): sc-100291

QUICK LINKS

SEE ALSO...

References:

α1A-AR 抗体 (4D8) Specifications

替代产品名称

产品克隆名称

分类

Clonality

抗体同型

氨基酸

抗原表位靶标

抗原表位种属

产品反应性/检测

种属反应性/检测

其它哺乳动物反应性

其它非哺乳动物反应性

应用

瓶的信息

研究引文

推荐的二抗检测试剂

可选的偶联物/形式

How selective is this antibody for alpha-1A? We need to address the selectivity issue in light of Michel et al. (2009) &quot;How reliable are G-protein-coupled receptor antibodies?&quot; NAUNYN-SCHMIEDEBERGS ARCHIVES OF PHARMACOLOGY 379: 386-388.

For Western Blot, is it recommended to use denatured or non-denatured conditions with &alpha;1A-AR (4D8): sc-100291 antibody?

α_1A-AR 抗体 (4D8): sc-100291

α_1A-AR 抗体 (4D8) Specifications

How selective is this antibody for alpha-1A? We need to address the selectivity issue in light of Michel et al. (2009) "How reliable are G-protein-coupled receptor antibodies?" NAUNYN-SCHMIEDEBERGS ARCHIVES OF PHARMACOLOGY 379: 386-388.

For Western Blot, is it recommended to use denatured or non-denatured conditions with α1A-AR (4D8): sc-100291 antibody?