α Enolase 抗体 (9): sc-101513

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Datasheets

- alpha Enolase 抗体 9 是小鼠单克隆 IgG₁ κ，α Enolase抗体, 在2篇文献中引用，规格为200 µg/ml
- 免疫human物种的重组α Enolase
- alpha Enolase 抗体 (9) 推荐用于 WB, IP 和 ELISA，检测mouse, rat 和human 来源的 α Enolase
- 抗-alpha Enolase 抗体 (9) 可偶联 琼脂糖 用于 IP; HRP 用于 WB, IHC(P) 和 ELISA; 还可偶联 藻红蛋白 或者 FITC 用于 IF, IHC(P) 和 FCM
- 还可偶联Alexa Fluor^® 488, Alexa Fluor^® 546, Alexa Fluor^® 594 和 Alexa Fluor^® 647，用于WB (RGB), IF, IHC(P) 和 FCM, 以及用于RGB荧光成像系统，例如iBright™ FL1000, FluorChem™, Typhoon, Azure和其他类似的系统
- 还可偶联Alexa Fluor^® 680 和 Alexa Fluor^® 790，用于WB (NIR), IF 和 FCM; 以及用于近红外（NIR）检测系统，如LI-COR^®/Odyssey^®, iBright™ FL1000, FluorChem™, Typhoon, Azure和类似系统
- 关于如何获取α Enolase (9): sc-101513的免费10 µg小样，联系我们技术服务部门 (或者您当地的代理商)了解详情。
- m-IgG₁ BP-HRP 是α Enolase Antibody (9) for WB首选应用二抗. 这些试剂现在以套装形式提供α Enolase Antibody (9) (见下方订购信息).

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此外 Enolase 抗体包括 α Enolase, β Enolase和 γ Enolase
Learn more about our ImmunoCruz® Antibody Conjugates and Cruz Marker™ MW Standards Promotional

α Enolase Antibody (9) - Western Blotting - Image 354504

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α Enolase Antibody (9) - Western Blotting - Image 378008

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α Enolase Antibody (9) is an IgG₁ κ mouse monoclonal alpha Enolase antibody (also designated ENO1 antibody, Alpha-enolase antibody or Enolase 1 antibody) that detects the alpha Enolase protein of mouse, rat and human origin by WB, IP and ELISA. α Enolase Antibody (9) is available as both the non-conjugated anti-alpha Enolase antibody form, as well as multiple conjugated forms of anti-alpha Enolase antibody, including agarose, HRP, PE, FITC and multiple Alexa Fluor^® conjugates. Enolases have been characterized as highly conserved cytoplasmic glycolytic enzymes that may be involved in differentiation. Three isoenzymes have been identified: α enolase, β enolase and γ enolase. α enolase expression has been detected on most tissues, whereas β enolase is expressed predominantly in muscle tissue and γ enolase is detected only in nervous tissue. These isoforms exist as both homodimers and heterodimers, and they play a role in converting phosphoglyceric acid to phosphenolpyruvic acid in the glycolytic pathway.

For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.

Alexa Fluor^® is a trademark of Molecular Probes Inc., OR., USA

LI-COR^® and Odyssey^® are registered trademarks of LI-COR Biosciences

种属	基因名称	基因ID	染色质位置	亚型（mRNA）即位号码	蛋白质即位号码	OMIM™ 号码
Human	ENO1	Entrez Gene ID: 2023	1p36.23	XM_006710433, NM_001201483, NM_001428	UniProt ID: P06733	n/a
Mouse	Eno1	Entrez Gene ID: 13806	4 E2	XM_006538525, XM_017319953, NM_023119	UniProt ID: P17182	N/A

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针对哺乳动物和非哺乳动物蛋白靶点的单克隆抗体基本上代表了多克隆抗体覆盖的所有靶点。我们提供超过8300种ImmunoCruz^®偶联抗体，从而可以省去使用二抗。

圣克鲁斯生物技术的抗体有超过36万的研究引文。抗α Enolase抗体(9)在各种科学出版物中有2篇引文。

针对哺乳动物靶标蛋白的一抗，如抗α Enolase 抗体 (9)，推荐用于检测一系列哺乳动物物种，主要有小鼠，大鼠和人物种。许多哺乳动物抗体也非常适合兽医研究，与羊、猪、羊、猫、犬、牛和马的蛋白靶点反应。圣克鲁斯生物技术的单克隆一抗适用于实验室研究应用，不用于诊断或治疗。

α Enolase 抗体 (9) Specifications

替代产品名称

ENO1

产品克隆名称

分类

一抗

Clonality

单克隆抗体

抗体同型

IgG₁ kappa light chain

氨基酸

抗原表位靶标

α Enolase

抗原表位种属

human

产品反应性/检测

α Enolase

种属反应性/检测

mouse, rat, human

其它哺乳动物反应性

N/A

其它非哺乳动物反应性

N/A

应用

WB, IP, ELISA

瓶的信息

Each vial contains 200 µg IgG₁ kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

研究引文

2篇各种科学出版物的引文

可选的偶联物/形式

AC: Agarose AF488: Alexa Fluor^® 488 AF546: Alexa Fluor^® 546 AF594: Alexa Fluor^® 594 AF647: Alexa Fluor^® 647 AF680: Alexa Fluor^® 680 AF790: Alexa Fluor^® 790 FITC: Fluoroscein HRP: Horseradish Peroxidase PE: Phycoerythrin

针对非哺乳动物靶点的一抗在检测植物、病毒、细菌、鸟类、鱼类、酵母和无脊椎动物的蛋白质方面非常有用。例如果蝇,斑马鱼, 非洲爪蟾蜍, 拟南芥, 秀丽隐杆线虫, 梭菌, 弯曲杆菌, 幽门螺杆菌, 肝炎, HIV, HPV, HSV, 流行性感冒, 克雷白氏杆菌, 结核分支杆菌, 疟原虫, 假单胞菌, RSV, 酿酒酵母, 沙门氏菌, 血吸虫, 志贺氏杆菌 和 葡萄球菌.

圣克鲁斯生物技术单克隆抗体可用于流式细胞术、免疫沉淀、Western blot和免疫染色等分析。抗α Enolase抗体(9)推荐用于以下应用：WB (Western blotting), IP (Immunoprecipitation), ELISA (Enzyme-Linked Immunosorbent Assay).

我们还有小鼠二抗、对照血清和对照免疫球蛋白，可与我们的单克隆一抗联合使用。

组织提取物，核提取物和全细胞裂解物与圣克鲁斯生物技术单克隆抗体使用，作为western blotting或免疫沉淀阳性对照。核提取物是理想的western blot分析或凝胶移位分析阳性对照。磷酸化富集的全细胞裂解液和组织提取物可用于磷酸化特异性抗体阳性对照。

单克隆二抗有多种偶联形式，包括：Biotin (B), CruzFluor™ 790, 680, 647, 594, 555, and 488, FITC, HRP, PerCP, PerCP-Cy5.5, Texas Red (TR), 和TRITC (tetramethyl rhodamine isothiocyanate) (R)，用于研究应用，像流式细胞术（FCM），免疫荧光（IF），免疫组织化学（IHC）染色和蛋白印迹（WB）。不用于诊断或治疗。

偶联和非偶联的亲和纯化的免疫球蛋白(IgGs)，用于流式细胞术（FCM），免疫荧光（IF），免疫组织化学（IHC），免疫沉淀（IP）和蛋白印迹（WB）应用，作为阴性对照。

正常血清用于免疫组织化学（IHC）应用，作为封闭试剂。所使用的血清应与二抗的种属相匹配。

優選的 α Enolase 抗體是 α Enolase 抗體 (L-27): sc-100812.

Does this antibody is good for Flow cytometry? which cells can I use to show positive control

Asked by: sigalpo

Thank you for your question. The antibody conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 is recommended for Flow cytometry. Hela and A549 cells are good positive controls for the α Enolase protein.

Answered by: Tech Service

Date published: 2020-02-04

Are there any issues with using sc-101513: α Enolase (9) mouse monoclonal primary antibody on mouse samples?

Asked by: TinTin

Thank you for your inquiry. The use of mouse monoclonal antibodies with mouse samples is very common and typically poses no problem. To eliminate any potential cross-reactivity of an anti-mouse secondary detection reagent,we recommend using a directly conjugated primary antibody. Please contact our special orders department for pricing and availability of sc-101513 conjugates.

Answered by: Technical Support

Date published: 2017-03-07

Rated 5 out of 5 by Stevie from Western blotting of human tissues Alpha-enolase alpha Enolase Antibody sc-101513 works well for western blot analysis of human salivary gland tissues.

Date published: 2021-02-13

Rated 5 out of 5 by MS from Good specificity in WB in HeLa whole cell lysate Good specificity in WB in HeLa whole cell lysate. -SCBT QC

Date published: 2015-04-03

Rated 5 out of 5 by ET from Strong band in WB in human recombinant Enolase Strong band in WB in human recombinant Enolase. -SCBT QC

Date published: 2014-06-27

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α Enolase 抗体 (9): sc-101513

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α Enolase 抗体 (9) Specifications

替代产品名称

产品克隆名称

分类

Clonality

抗体同型

氨基酸

抗原表位靶标

抗原表位种属

产品反应性/检测

种属反应性/检测

其它哺乳动物反应性

其它非哺乳动物反应性

应用

瓶的信息

研究引文

推荐的二抗检测试剂

可选的偶联物/形式

優選的 α Enolase 抗體是 α Enolase 抗體 (L-27): sc-100812.

Does this antibody is good for Flow cytometry? which cells can I use to show positive control

Are there any issues with using sc-101513: α Enolase (9) mouse monoclonal primary antibody on mouse samples?

α Enolase 抗体 (9): sc-101513

QUICK LINKS

SEE ALSO...

α Enolase 抗体 (9) Specifications

替代产品名称

产品克隆名称

分类

Clonality

抗体同型

氨基酸

抗原表位靶标

抗原表位种属

产品反应性/检测

种属反应性/检测

其它哺乳动物反应性

其它非哺乳动物反应性

应用

瓶的信息

研究引文

推荐的二抗检测试剂

可选的偶联物/形式

優選的 α Enolase 抗體是 α Enolase 抗體 (L-27): sc-100812.

Does this antibody is good for Flow cytometry? which cells can I use to show positive control

Are there any issues with using sc-101513: &alpha; Enolase (9) mouse monoclonal primary antibody on mouse samples?

Are there any issues with using sc-101513: α Enolase (9) mouse monoclonal primary antibody on mouse samples?