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possible, and a separate shipping charge will be included for each shipping method required. Shipping charges listed below are from our US warehouses to the Contiguous US,
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SHIPPING METHODS & CHARGES
Ships via FedEx Ground to Contiguous US, Alaska, Canada, Monday through Friday. This method is used for less temperature sensitive items such as lab ware and animal
health products, bulky and/or heavy items
Labware ships FedEx Ground free of charge to the contiguous US
2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD) is the prototype for a family of toxic halogenated aromatic compounds that are thought to cause adverse reproductive, immunologic and metabolic effects. Many biological responses to TCDD are mediated through ligand binding to the aromatic hydrocarbon (Ah) receptor, also known as AhR. Ah Receptor is a ligand dependent transcription factor that interacts with specific DNA sequences, termed xenobiotic responsive elements (XREs), and that lies upstream of TCDD-inducible genes. Upon binding to the ligand, Ah Receptor binds to the Ah Receptor nuclear translocator (Arnt), and the complex is translocated from the cytoplasm to the nucleus. Arnt is required for Ah R eceptor to bind to XRE. Ah Receptor and Arnt are members of a family of transcription factors that contain a basic helix-loop-helix motif and a common "PAS" motif.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.
I will use Ah Receptor (L-15) : sc-101104 for WB in Human fibroblast cells, what dilution of this antibody should I use and what is the best, BSA or milk for blocking ? What kind of membrane should be the best to reveal the Ah Receptor ?
Asked by: Cells
Thank you for your question. The recommended starting dilution for this antibody is 1:200, with a dilution range of 1:100-1:1000. We recommend using UltraCruz® Blocking Reagent: sc-516214 for blocking, which is also the reagent that is used to establish our antibodies before they are released.
It is also possible to block with 5 % BSA TBST (if binding is promiscuous) or 5 % non-fatty milk TBST (if binding is rather weak).
For further information, please contact our technical service.
Answered by: SCBT Heidelberg Support
Date published: 2019-01-06
I am using Ah Receptor (L-15): sc-101104 for immunofluorescence, what dilution of this antibody should I use?
Asked by: DefinitelyNotMatt
Thank you for your question. We recommend a starting dilution of 1:50. However, optimal antibody concentration should be determined by titration. Please find our full immunofluorescence protocol here: