Ah Receptor抗体 (L-15) 推荐用于 mouse, rat 和 human 来源的Ah Receptor WB, IP, IF 和 ELISA检测

Ah Receptor 抗体 (L-15): sc-101104

Ah Receptor抗体 (L-15) is rated 4.0 out of 5 by 3.
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Datasheets

    • Ah Receptor抗体(L-15)是小鼠单克隆IgG1 (kappa轻链),规格为100 µg/ml
    • 免疫human物种的重组Ah Receptor
    • 推荐用于 mouse, rat 和 human 来源的Ah Receptor WB, IP, IF 和 ELISA检测
    • 查看我们强烈推荐的传统抗小鼠IgG二抗的重组替代品 m-IgGκ BP-HRP (mouse IgGκ binding protein-HRP)
    • 看 Ah Receptor (A-3): sc-133088,可查看有标记的 Ah Receptor 抗体,包括AC, HRP, FITC, PE, Alexa Fluor® 488, 594, 647, 680 和 790

I will use Ah Receptor (L-15) : sc-101104 for WB in Human fibroblast cells, what dilution of this antibody should I use and what is the best, BSA or milk for blocking ? What kind of membrane should be the best to reveal the Ah Receptor ?

Asked by: Cells
Thank you for your question. The recommended starting dilution for this antibody is 1:200, with a dilution range of 1:100-1:1000. We recommend using UltraCruz® Blocking Reagent: sc-516214 for blocking, which is also the reagent that is used to establish our antibodies before they are released. It is also possible to block with 5 % BSA TBST (if binding is promiscuous) or 5 % non-fatty milk TBST (if binding is rather weak). For further information, please contact our technical service.
Answered by: SCBT Heidelberg Support
Date published: 2019-01-06

I am using Ah Receptor (L-15): sc-101104 for immunofluorescence, what dilution of this antibody should I use?

Asked by: DefinitelyNotMatt
Thank you for your question. We recommend a starting dilution of 1:50. However, optimal antibody concentration should be determined by titration. Please find our full immunofluorescence protocol here: https://www.scbt.com/scbt/resources/protocols/immunofluorescence-cell-staining
Answered by: Technical Support
Date published: 2017-03-24
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Rated 4 out of 5 by from Good immunofluorescence nuclear staining Good immunofluorescence nuclear staining in paraformaldehyde-fixed HeLa cells. -SCBT QC
Date published: 2015-05-20
Rated 4 out of 5 by from Great WB signal achieved in human Ah Receptor Great WB signal achieved in human Ah Receptor transfected 293T whole cell lysate. -SCBT QC
Date published: 2015-04-20
Rated 4 out of 5 by from Very precise signal obtained in WB with COLO Very precise signal obtained in WB with COLO 320 HSR whole cell lysate. -SCBT QC
Date published: 2015-04-14
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