Ah Receptor 抗体 (L-15): sc-101104
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- Ah Receptor抗体(L-15)是小鼠单克隆IgG1 κ, 在5篇文献中引用，规格为100 µg/ml
- 免疫human物种的重组Ah Receptor
- 推荐用于 mouse, rat 和 human 来源的Ah Receptor WB, IP, IF 和 ELISA检测
- 看 Ah Receptor (A-3): sc-133088，可查看有标记的 Ah Receptor 抗体，包括AC, HRP, FITC, PE, Alexa Fluor® 488, 594, 647, 680 和 790
- 目前，我们还没有完成Ah Receptor 抗体 (L-15)的首选二抗检测试剂的鉴定。这项工作正在进行中。
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SEE ALSO...
- ChemCruz™ Chemical Aryl Hydrocarbon Receptor Inhibitors or Aryl Hydrocarbon Receptor Activators可用于Aryl Hydrocarbon Receptor细胞反应的功能性分析
I will use Ah Receptor (L-15) : sc-101104 for WB in Human fibroblast cells, what dilution of this antibody should I use and what is the best, BSA or milk for blocking ? What kind of membrane should be the best to reveal the Ah Receptor ?
Thank you for your question. The recommended starting dilution for this antibody is 1:200, with a dilution range of 1:100-1:1000. We recommend using UltraCruz® Blocking Reagent: sc-516214 for blocking, which is also the reagent that is used to establish our antibodies before they are released.
It is also possible to block with 5 % BSA TBST (if binding is promiscuous) or 5 % non-fatty milk TBST (if binding is rather weak).
For further information, please contact our technical service.
Date published: 2019-01-06
I am using Ah Receptor (L-15): sc-101104 for immunofluorescence, what dilution of this antibody should I use?
Thank you for your question. We recommend a starting dilution of 1:50. However, optimal antibody concentration should be determined by titration. Please find our full immunofluorescence protocol here:
https://www.scbt.com/scbt/resources/protocols/immunofluorescence-cell-staining
Date published: 2017-03-24
Rated 4 out of
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TS from
Good immunofluorescence nuclear staining
Good immunofluorescence nuclear staining in paraformaldehyde-fixed HeLa cells. -SCBT QC
Date published: 2015-05-20
Rated 4 out of
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SF from
Great WB signal achieved in human Ah Receptor
Great WB signal achieved in human Ah Receptor transfected 293T whole cell lysate. -SCBT QC
Date published: 2015-04-20
Rated 4 out of
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Very precise signal obtained in WB with COLO
Very precise signal obtained in WB with COLO 320 HSR whole cell lysate. -SCBT QC
Date published: 2015-04-14