Thank you for your question. The recommended starting dilution for this antibody is 1:200, with a dilution range of 1:100-1:1000. We recommend using UltraCruz® Blocking Reagent: sc-516214 for blocking, which is also the reagent that is used to establish our antibodies before they are released.
It is also possible to block with 5 % BSA TBST (if binding is promiscuous) or 5 % non-fatty milk TBST (if binding is rather weak).
For further information, please contact our technical service.
Date published: 2019-01-06