Act1抗体 (WW-18) 推荐用于 mouse, rat 和 human 来源的Act1 WB, IP, IF, IHC(P) 和 ELISA检测

Act1 抗体 (WW-18): sc-100647

Act1抗体 (WW-18) is rated 5.0 out of 5 by 6.
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Datasheets

    • Act1抗体(WW-18)是小鼠单克隆IgG2a (kappa轻链),规格为100 µg/ml
    • 免疫human物种的重组Act1
    • 推荐用于 mouse, rat 和 human 来源的Act1 WB, IP, IF, IHC(P) 和 ELISA检测
    • 查看我们强烈推荐的传统抗小鼠IgG二抗的重组替代品 m-IgGκ BP-HRP (mouse IgGκ binding protein-HRP)
    • 关于如何获取Act1 (WW-18): sc-100647免费10 µg小样,联系我们技术服务部门 (或者您当地的代理商)了解详情。
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Hi, I bought a act-1 antibody from your company. Do you know the recognition region of this antibody? Thank you

Asked by: Weifeng
Thank you for your question. This antibody was raised against recombinant Act1 of human origin. The exact binding epitope has not been mapped. If you have any further questions, please contact your local distributor, or technical service
Answered by: Tech Service
Date published: 2019-10-31

What is the recommended antigen retrieval for this antibody when doing IHC with paraffin-embedded sections?

Asked by: AbPolly
Thank you for your question. We recommend sodium citrate buffer, pH 6.0, with heat for the antigen retrieval step. The full protocol can be found here: https://www.scbt.com/scbt/resources/protocols/immunoperoxidase-staining
Answered by: Technical Support 4
Date published: 2017-01-18
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Rated 5 out of 5 by from Good product Worked very well in western blotting. Tested using HepG2 lysate and showed very linear changes with 2 fold dilutions (20 to 2.5 ug/well)
Date published: 2019-08-08
Rated 5 out of 5 by from good antibody good antibody and you can get very clear result in your wb.
Date published: 2017-11-21
Rated 5 out of 5 by from Outstanding Very nice western blot results with strong clear signal; also strong IF and IHC staining data
Date published: 2017-02-01
Rated 5 out of 5 by from Excellent! This antibody is great in western blot analysis of Act1 expression in Hep G2 nuclear extract.
Date published: 2017-01-10
Rated 5 out of 5 by from Produce nice HRP Produce nice HRP-DAB staining, Parafin section- Sodium Citrate PH6.0 bioling water 20 min; 4 degree 1:50 overnight; Dako Goat Anti mouse-biotin 1:500 ; then Vector strepavdin-HRP 1:200. Human skin tissue.
Date published: 2015-11-18
Rated 5 out of 5 by from Produced beautiful immunoperoxidase membrane Produced beautiful immunoperoxidase membrane and cytoplasmic staining in formalin-fixed, paraffin-embedded human small Intestine tissue. -SCBT QC
Date published: 2015-05-17
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