Act1 抗体 (WW-18): sc-100647

5.0(6)

- Act1抗体(WW-18)是小鼠单克隆IgG_2a κ, 在11篇文献中引用，规格为100 µg/ml
- 免疫human物种的重组Act1
- 推荐用于 mouse, rat 和 human 来源的Act1 WB, IP, IF, IHC(P) 和 ELISA检测
- 关于如何获取Act1 (WW-18): sc-100647的免费10 µg小样，联系我们技术服务部门 (或者您当地的代理商)了解详情。
- m-IgG Fc BP-HRP and m-IgG_2a BP-HRP 是 Act1 Antibody (WW-18) for WB and IHC(P)首选应用二抗。这些试剂现在以套装形式提供Act1 Antibody (WW-18) (见下方订购信息).

References:

Phosphorylation meets ubiquitination: the control of NF-[kappa]B activity. | Karin, M. and Ben-Neriah, Y. 2000. Annu Rev Immunol. 18: 621-63. PMID: 10837071
Act1, an NF-kappa B-activating protein. | Li, X., et al. 2000. Proc Natl Acad Sci U S A. 97: 10489-93. PMID: 10962024
CIKS, a connection to Ikappa B kinase and stress-activated protein kinase. | Leonardi, A., et al. 2000. Proc Natl Acad Sci U S A. 97: 10494-9. PMID: 10962033
Role of the adaptor protein CIKS in the activation of the IKK complex. | Mauro, C., et al. 2003. Biochem Biophys Res Commun. 309: 84-90. PMID: 12943667
Requirement for both JAK-mediated PI3K signaling and ACT1/TRAF6/TAK1-dependent NF-kappaB activation by IL-17A in enhancing cytokine expression in human airway epithelial cells. | Huang, F., et al. 2007. J Immunol. 179: 6504-13. PMID: 17982039
Act1 modulates autoimmunity through its dual functions in CD40L/BAFF and IL-17 signaling. | Li, X. 2008. Cytokine. 41: 105-13. PMID: 18061473
Structure, regulation and function of NF-kappa B. | Siebenlist, U., et al. 1994. Annu Rev Cell Biol. 10: 405-55. PMID: 7888182
Nuclear factor-kappaB: a pivotal transcription factor in chronic inflammatory diseases. | Barnes, PJ. and Karin, M. 1997. N Engl J Med. 336: 1066-71. PMID: 9091804

种属	基因名称	基因ID	染色质位置	亚型（mRNA）即位号码	蛋白质即位号码	OMIM™ 号码
Human	TRAF3IP2	Entrez Gene ID: 10758	6q21	XM_006715319, XM_011535386, NM_001164281, NM_001164283, NM_147200, NM_147686, NM_001164282	UniProt ID: O43734	607043
Mouse	Traf3ip2	Entrez Gene ID: 103213	10 B1	XM_011243105, NM_134000	UniProt ID: XP_011241407	N/A

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Protocols Online

针对哺乳动物和非哺乳动物蛋白靶点的单克隆抗体基本上代表了多克隆抗体覆盖的所有靶点。我们提供超过8300种ImmunoCruz^®偶联抗体，从而可以省去使用二抗。

圣克鲁斯生物技术的抗体有超过36万的研究引文。抗Act1抗体(WW-18)在各种科学出版物中有11篇引文。

针对哺乳动物靶标蛋白的一抗，如抗Act1 抗体 (WW-18)，推荐用于检测一系列哺乳动物物种，主要有小鼠，大鼠和人物种。许多哺乳动物抗体也非常适合兽医研究，与羊、猪、羊、猫、犬、牛和马的蛋白靶点反应。圣克鲁斯生物技术的单克隆一抗适用于实验室研究应用，不用于诊断或治疗。

Act1 抗体 (WW-18) Specifications

替代产品名称

Act1

产品克隆名称

WW-18

分类

一抗

Clonality

单克隆抗体

抗体同型

IgG_2a kappa light chain

氨基酸

451-566

抗原表位靶标

Act1

抗原表位种属

human

产品反应性/检测

Act1

种属反应性/检测

mouse, rat, human

其它哺乳动物反应性

N/A

其它非哺乳动物反应性

N/A

应用

WB, IP, IF, IHC(P), ELISA

瓶的信息

Each vial contains 100 µg IgG_2a kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

研究引文

11篇各种科学出版物的引文

可选的偶联物/形式

N/A

针对非哺乳动物靶点的一抗在检测植物、病毒、细菌、鸟类、鱼类、酵母和无脊椎动物的蛋白质方面非常有用。例如果蝇,斑马鱼, 非洲爪蟾蜍, 拟南芥, 秀丽隐杆线虫, 梭菌, 弯曲杆菌, 幽门螺杆菌, 肝炎, HIV, HPV, HSV, 流行性感冒, 克雷白氏杆菌, 结核分支杆菌, 疟原虫, 假单胞菌, RSV, 酿酒酵母, 沙门氏菌, 血吸虫, 志贺氏杆菌 和 葡萄球菌.

圣克鲁斯生物技术单克隆抗体可用于流式细胞术、免疫沉淀、Western blot和免疫染色等分析。抗Act1抗体(WW-18)推荐用于以下应用：WB (Western blotting), IP (Immunoprecipitation), IF (Immunofluorescence), IHC(P) (Immunohistochemistry - Paraffin Embedded), ELISA (Enzyme-Linked Immunosorbent Assay).

我们还有小鼠二抗、对照血清和对照免疫球蛋白，可与我们的单克隆一抗联合使用。

组织提取物，核提取物和全细胞裂解物与圣克鲁斯生物技术单克隆抗体使用，作为western blotting或免疫沉淀阳性对照。核提取物是理想的western blot分析或凝胶移位分析阳性对照。磷酸化富集的全细胞裂解液和组织提取物可用于磷酸化特异性抗体阳性对照。

单克隆二抗有多种偶联形式，包括：Biotin (B), CruzFluor™ 790, 680, 647, 594, 555, and 488, FITC, HRP, PerCP, PerCP-Cy5.5, Texas Red (TR), 和TRITC (tetramethyl rhodamine isothiocyanate) (R)，用于研究应用，像流式细胞术（FCM），免疫荧光（IF），免疫组织化学（IHC）染色和蛋白印迹（WB）。不用于诊断或治疗。

偶联和非偶联的亲和纯化的免疫球蛋白(IgGs)，用于流式细胞术（FCM），免疫荧光（IF），免疫组织化学（IHC），免疫沉淀（IP）和蛋白印迹（WB）应用，作为阴性对照。

正常血清用于免疫组织化学（IHC）应用，作为封闭试剂。所使用的血清应与二抗的种属相匹配。

優選的 Act1 抗體是 Act1 抗體 (D-11): sc-398161.

Hi, I bought a act-1 antibody from your company. Do you know the recognition region of this antibody? Thank you

Asked by: Weifeng

Thank you for your question. This antibody was raised against recombinant Act1 of human origin. The exact binding epitope has not been mapped. If you have any further questions, please contact your local distributor, or technical service

Answered by: Tech Service

Date published: 2019-10-31

What is the recommended antigen retrieval for this antibody when doing IHC with paraffin-embedded sections?

Asked by: AbPolly

Thank you for your question. We recommend sodium citrate buffer, pH 6.0, with heat for the antigen retrieval step. The full protocol can be found here: https://www.scbt.com/scbt/resources/protocols/immunoperoxidase-staining

Answered by: Technical Support 4

Date published: 2017-01-18

Rated 5 out of 5 by Mido from Good product Worked very well in western blotting. Tested using HepG2 lysate and showed very linear changes with 2 fold dilutions (20 to 2.5 ug/well)

Date published: 2019-08-08

Rated 5 out of 5 by HAIPENG from good antibody good antibody and you can get very clear result in your wb.

Date published: 2017-11-21

Rated 5 out of 5 by Bill28 from Outstanding Very nice western blot results with strong clear signal; also strong IF and IHC staining data

Date published: 2017-02-01

Rated 5 out of 5 by jerojero from Excellent! This antibody is great in western blot analysis of Act1 expression in Hep G2 nuclear extract.

Date published: 2017-01-10

Rated 5 out of 5 by NW from Produce nice HRP Produce nice HRP-DAB staining, Parafin section- Sodium Citrate PH6.0 bioling water 20 min; 4 degree 1:50 overnight; Dako Goat Anti mouse-biotin 1:500 ; then Vector strepavdin-HRP 1:200. Human skin tissue.

Date published: 2015-11-18

Rated 5 out of 5 by SF from Produced beautiful immunoperoxidase membrane Produced beautiful immunoperoxidase membrane and cytoplasmic staining in formalin-fixed, paraffin-embedded human small Intestine tissue. -SCBT QC

Date published: 2015-05-17

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Act1 抗体 (WW-18): sc-100647

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References:

Act1 抗体 (WW-18) Specifications

替代产品名称

产品克隆名称

分类

Clonality

抗体同型

氨基酸

抗原表位靶标

抗原表位种属

产品反应性/检测

种属反应性/检测

其它哺乳动物反应性

其它非哺乳动物反应性

应用

瓶的信息

研究引文

推荐的二抗检测试剂

可选的偶联物/形式

優選的 Act1 抗體是 Act1 抗體 (D-11): sc-398161.

Hi, I bought a act-1 antibody from your company. Do you know the recognition region of this antibody? Thank you

What is the recommended antigen retrieval for this antibody when doing IHC with paraffin-embedded sections?