AATK抗体 (L-37) 推荐用于 mouse, rat 和 human 来源的AATK WB, IP, IF, IHC(P) 和 ELISA检测

AATK 抗体 (L-37): sc-100436

AATK抗体 (L-37) is rated 4.3 out of 5 by 4.
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Datasheets

    • 抗-AATK 抗体 L-37 是小鼠单克隆 IgG1 (kappa轻链),AATK抗体,规格为50 µg/0.5 ml
    • 免疫human物种的重组AATK
    • 推荐用于 mouse, rat 和 human 来源的AATK WB, IP, IF, IHC(P) 和 ELISA检测
    • 查看我们强烈推荐的传统抗小鼠IgG二抗的重组替代品 m-IgGκ BP-HRP (mouse IgGκ binding protein-HRP)
    • 关于如何获取AATK (L-37): sc-100436免费10 µg小样,联系我们技术服务部门 (或者您当地的代理商)了解详情。
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why the size of data sheet for this antibody is 145 kDa but the western blot picture showed from 86-110 kDa ? any explanation ?

Asked by: ody82
Thank you for your question. According to Uniprot (see below), AATK has 3 isoforms with predicted molecular weights of 145 kDa, 45 kDa and 93 kDa. This antibody would not be able to recognize isoform 2 (missing amino acids 1-433). Based on the observed molecular weights alone, this antibody may recognize both isoform 1 and 3 in the cell lines tested. https://www.uniprot.org/uniprot/Q6ZMQ8#sequences
Answered by: Technical Support
Date published: 2019-05-03

Can sc-100436: AATK (L-37) monoclonal antibody be used to stain formalin-fixed, paraffin-embedded (FFPE) tissue sections?

Asked by: Professor Griffin
Thank you for your question. Yes, sc-100436: AATK (L-37) is recommended for use in IHC with paraffin-embedded sections. We recommend performing antigen retrieval with sodium citrate buffer (pH 6) and heat. The full protocol can be found here: https://www.scbt.com/scbt/resources/protocols/immunoperoxidase-staining
Answered by: Technical Support
Date published: 2017-03-24
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Rated 4 out of 5 by from Good in WB, questionable species cross reactivity Works well in untransfected HEK293 lysate, single clean band of the correct size when used at 1:250 in 1% milk. However, nothing in whole brain lysate. LMTK1 is clearly enriched in neurons as per multiple literature citations. Maybe the antibody does not cross react with mouse and only recognizes human protein?
Date published: 2018-04-13
Rated 5 out of 5 by from good Ab for human sample i bought this Ab a month ago and i did western blot in human tissues. the signal was great.
Date published: 2018-04-11
Rated 4 out of 5 by from Great immunoperoxidase membrane and cytoplasmic Great immunoperoxidase membrane and cytoplasmic staining in formalin-fixed, paraffin-embedded human stomach tissue. -SCBT QC
Date published: 2015-03-16
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