AATK 抗体 (L-37): sc-100436

4.2(4)

- AATK 抗体 L-37 是小鼠单克隆 IgG₁ (kappa轻链)，AATK抗体，规格为50 µg/0.5 ml
- 免疫human物种的重组AATK
- 推荐用于 mouse, rat 和 human 来源的AATK WB, IP, IF, IHC(P) 和 ELISA检测
- 关于如何获取AATK (L-37): sc-100436的免费10 µg小样，联系我们技术服务部门 (或者您当地的代理商)了解详情。
- 目前，我们还没有完成AATK 抗体 (L-37)的首选二抗检测试剂的鉴定。这项工作正在进行中。

References:

Chromosomal assignment of a human apoptosis-associated tyrosine kinase gene on chromosome 17q25.3 by somatic hybrid analysis and fluorescence in situ hybridization. | Seki, N., et al. 1999. J Hum Genet. 44: 141-2. PMID: 10083745
A novel kinase, AATYK induces and promotes neuronal differentiation in a human neuroblastoma (SH-SY5Y) cell line. | Raghunath, M., et al. 2000. Brain Res Mol Brain Res. 77: 151-62. PMID: 10837911
Characterization of an alternatively spliced AATYK mRNA: expression pattern of AATYK in the brain and neuronal cells. | Baker, SJ., et al. 2001. Oncogene. 20: 1015-21. PMID: 11314039
Characterization of the apoptosis-associated tyrosine kinase (AATYK) expressed in the CNS. | Tomomura, M., et al. 2001. Oncogene. 20: 1022-32. PMID: 11314040
Differential expression and function of apoptosis-associated tyrosine kinase (AATYK) in the developing mouse brain. | Tomomura, M., et al. 2003. Brain Res Mol Brain Res. 112: 103-12. PMID: 12670708
Apoptosis-associated tyrosine kinase (AATYK) has differential Ca2+-dependent phosphorylation states in response to survival and apoptotic conditions in cerebellar granule cells. | Tomomura, M. and Furuichi, T. 2005. J Biol Chem. 280: 35157-63. PMID: 16100393

种属	基因名称	基因ID	染色质位置	亚型（mRNA）即位号码	蛋白质即位号码	OMIM™ 号码
Human	AATK	Entrez Gene ID: 9625	17q25.3	XM_006722193, XM_006722194, XM_006722195, XM_006722196, XM_011525509, XM_011525510, XM_011525511, XM_017025421, XM_017025422, NM_001080395, NM_004920	UniProt ID: Q6ZMQ8
Mouse	Aatk	Entrez Gene ID: 11302	11 E2	XM_006531991, XM_006531992, XM_006531993, XM_006531994, XM_006531995, NM_001198785, NM_001198787, NM_007377	UniProt ID: Q80YE4	N/A

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针对哺乳动物和非哺乳动物蛋白靶点的单克隆抗体基本上代表了多克隆抗体覆盖的所有靶点。我们提供超过8300种ImmunoCruz^®偶联抗体，从而可以省去使用二抗。

圣克鲁斯生物技术的抗体有超过36万的研究引文。抗AATK抗体(L-37)在各种科学出版物中有0篇引文。

针对哺乳动物靶标蛋白的一抗，如抗AATK 抗体 (L-37)，推荐用于检测一系列哺乳动物物种，主要有小鼠，大鼠和人物种。许多哺乳动物抗体也非常适合兽医研究，与羊、猪、羊、猫、犬、牛和马的蛋白靶点反应。圣克鲁斯生物技术的单克隆一抗适用于实验室研究应用，不用于诊断或治疗。

AATK 抗体 (L-37) Specifications

替代产品名称

AATK

产品克隆名称

L-37

分类

一抗

Clonality

单克隆抗体

抗体同型

IgG₁ kappa light chain

氨基酸

161-260

抗原表位靶标

AATK

抗原表位种属

human

产品反应性/检测

AATK

种属反应性/检测

mouse, rat, human

其它哺乳动物反应性

N/A

其它非哺乳动物反应性

N/A

应用

WB, IP, IF, IHC(P), ELISA

瓶的信息

Each vial contains 50 µg IgG₁ kappa light chain in 0.5 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

研究引文

0篇各种科学出版物的引文

可选的偶联物/形式

N/A

针对非哺乳动物靶点的一抗在检测植物、病毒、细菌、鸟类、鱼类、酵母和无脊椎动物的蛋白质方面非常有用。例如果蝇,斑马鱼, 非洲爪蟾蜍, 拟南芥, 秀丽隐杆线虫, 梭菌, 弯曲杆菌, 幽门螺杆菌, 肝炎, HIV, HPV, HSV, 流行性感冒, 克雷白氏杆菌, 结核分支杆菌, 疟原虫, 假单胞菌, RSV, 酿酒酵母, 沙门氏菌, 血吸虫, 志贺氏杆菌 和 葡萄球菌.

圣克鲁斯生物技术单克隆抗体可用于流式细胞术、免疫沉淀、Western blot和免疫染色等分析。抗AATK抗体(L-37)推荐用于以下应用：WB (Western blotting), IP (Immunoprecipitation), IF (Immunofluorescence), IHC(P) (Immunohistochemistry - Paraffin Embedded), ELISA (Enzyme-Linked Immunosorbent Assay).

我们还有小鼠二抗、对照血清和对照免疫球蛋白，可与我们的单克隆一抗联合使用。

组织提取物，核提取物和全细胞裂解物与圣克鲁斯生物技术单克隆抗体使用，作为western blotting或免疫沉淀阳性对照。核提取物是理想的western blot分析或凝胶移位分析阳性对照。磷酸化富集的全细胞裂解液和组织提取物可用于磷酸化特异性抗体阳性对照。

单克隆二抗有多种偶联形式，包括：Biotin (B), CruzFluor™ 790, 680, 647, 594, 555, and 488, FITC, HRP, PerCP, PerCP-Cy5.5, Texas Red (TR), 和TRITC (tetramethyl rhodamine isothiocyanate) (R)，用于研究应用，像流式细胞术（FCM），免疫荧光（IF），免疫组织化学（IHC）染色和蛋白印迹（WB）。不用于诊断或治疗。

偶联和非偶联的亲和纯化的免疫球蛋白(IgGs)，用于流式细胞术（FCM），免疫荧光（IF），免疫组织化学（IHC），免疫沉淀（IP）和蛋白印迹（WB）应用，作为阴性对照。

正常血清用于免疫组织化学（IHC）应用，作为封闭试剂。所使用的血清应与二抗的种属相匹配。

why the size of data sheet for this antibody is 145 kDa but the western blot picture showed from 86-110 kDa ? any explanation ?

Asked by: ody82

Thank you for your question. According to Uniprot (see below), AATK has 3 isoforms with predicted molecular weights of 145 kDa, 45 kDa and 93 kDa. This antibody would not be able to recognize isoform 2 (missing amino acids 1-433). Based on the observed molecular weights alone, this antibody may recognize both isoform 1 and 3 in the cell lines tested. https://www.uniprot.org/uniprot/Q6ZMQ8#sequences

Answered by: Technical Support

Date published: 2019-05-03

Can sc-100436: AATK (L-37) monoclonal antibody be used to stain formalin-fixed, paraffin-embedded (FFPE) tissue sections?

Asked by: Professor Griffin

Thank you for your question. Yes, sc-100436: AATK (L-37) is recommended for use in IHC with paraffin-embedded sections. We recommend performing antigen retrieval with sodium citrate buffer (pH 6) and heat. The full protocol can be found here: https://www.scbt.com/scbt/resources/protocols/immunoperoxidase-staining

Answered by: Technical Support

Date published: 2017-03-24

Rated 4 out of 5 by StanK from Good in WB, questionable species cross reactivity Works well in untransfected HEK293 lysate, single clean band of the correct size when used at 1:250 in 1% milk. However, nothing in whole brain lysate. LMTK1 is clearly enriched in neurons as per multiple literature citations. Maybe the antibody does not cross react with mouse and only recognizes human protein?

Date published: 2018-04-13

Rated 5 out of 5 by Anonymous from good Ab for human sample i bought this Ab a month ago and i did western blot in human tissues. the signal was great.

Date published: 2018-04-11

Rated 4 out of 5 by FL from Great immunoperoxidase membrane and cytoplasmic Great immunoperoxidase membrane and cytoplasmic staining in formalin-fixed, paraffin-embedded human stomach tissue. -SCBT QC

Date published: 2015-03-16

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AATK 抗体 (L-37): sc-100436

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References:

AATK 抗体 (L-37) Specifications

替代产品名称

产品克隆名称

分类

Clonality

抗体同型

氨基酸

抗原表位靶标

抗原表位种属

产品反应性/检测

种属反应性/检测

其它哺乳动物反应性

其它非哺乳动物反应性

应用

瓶的信息

研究引文

推荐的二抗检测试剂

可选的偶联物/形式

why the size of data sheet for this antibody is 145 kDa but the western blot picture showed from 86-110 kDa ? any explanation ?

Can sc-100436: AATK (L-37) monoclonal antibody be used to stain formalin-fixed, paraffin-embedded (FFPE) tissue sections?