60 kDa Ro/SSA 抗体 (AA-3): sc-100844

60 kDa Ro/SSA Antibody (AA-3) is an IgG_2a κ mouse monoclonal 60 kDa Ro/SSA antibody (also designated 60 kDa Ro/SSA antibody) suitable for the detection of the 60 kDa Ro/SSA protein of mouse, rat and human origin by WB, IP, IF and ELISA. 60 kDa Ro/SSA Antibody (AA-3) is available as the non-conjugated anti-60 kDa Ro/SSA antibody. Ro autoantigens are of clinical significance because antibodies directed against them are found in most patients with primary Sjogren syndrome, subacute cutaneous lupus erythematosus (SLE), neonatal lupus erythematosus, ANA-negative lupus erythematosus, and systemic lupus erythematosus-like disease secondary to homozygous C2 or C4 complement deficiency. Ro/SSA is a ribonucleoprotein that binds to autoantibodies in 35 to 50% of patients with SLE and in up to 97% of patients with Sjogren syndrome. The Ro/SSA particle consists of a single immunoreactive protein noncovalently bound with one of four small RNA molecules. Most anti-Ro/SSA-positive sera antibodies detect not only the main protein, but also a smaller Ro/SSA protein. The genes which encode the smaller and larger proteins map to human chromosomes 11p15.5 and 1q31, respectively. La/SSB is an autoimmune RNA-binding protein that plays a role in the transcription of RNA polymerase III was originally defined by its reactivity with autoantibodies from patients with Sjogren syndrome and SLE.

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References:

1. Autoantibody to the leucine zipper region of 52 kDa Ro/SSA binds native 60 kDa Ro/SSA: identification of a tertiary epitope with components from 60 kDa Ro/SSA and 52 kDa Ro/SSA.  |  Kurien, BT., et al. 2001. Scand J Immunol. 53: 268-76. PMID: 11251884
2. Differential expression of Ro/SSA 60 kDa and La/SSB, but not Ro/SSA 52 kDa, mRNA and protein in minor salivary glands from patients with primary Sjögren′s syndrome.  |  Barcellos, KS., et al. 2007. J Rheumatol. 34: 1283-92. PMID: 17552056
3. Human Y5 RNA specializes a Ro ribonucleoprotein for 5S ribosomal RNA quality control.  |  Hogg, JR. and Collins, K. 2007. Genes Dev. 21: 3067-72. PMID: 18056422
4. Protein heterogeneity in the human Ro/SSA ribonucleoproteins. The 52- and 60-kD Ro/SSA autoantigens are encoded by separate genes.  |  Itoh, K., et al. 1991. J Clin Invest. 87: 177-86. PMID: 1985094
5. Genomic structure and amino acid sequence domains of the human La autoantigen.  |  Chambers, JC., et al. 1988. J Biol Chem. 263: 18043-51. PMID: 3192525
6. Human 60-kDa SS-A/Ro ribonucleoprotein autoantigen gene (SSA2) localized to 1q31 by fluorescence in situ hybridization.  |  Chan, EK., et al. 1994. Genomics. 23: 298-300. PMID: 7829096
7. The mapping of the human 52-kD Ro/SSA autoantigen gene to human chromosome 11, and its polymorphisms.  |  Frank, MB., et al. 1993. Am J Hum Genet. 52: 183-91. PMID: 8094596