3β-HSD 抗体 (37-2): sc-100466

3β-HSD Antibody (37-2) is an IgG1 κ mouse monoclonal 3β-HSD antibody that detects 3β-HSD and 3β-HSD2 of human origin by WB, IP, IF, IHC(P) and ELISA. 3β-HSD Antibody (37-2) is available as the non-conjugated anti-3β-HSD antibody form. 3 beta-hydroxysteroid dehydrogenase (3β-HSD), also known as HSD3B1 or HSDB3, is a bifunctional enzyme that plays a crucial role in the synthesis of all classes of hormonal steroids. Two human 3β-HSD proteins, designated type I (3β-HSD) and type II (3β-HSD2), are expressed by different genes and function in different areas of the body. Localized to the membrane of the endoplasmic reticulum (ER) and expressed in skin and placenta, 3β-HSD is the type I protein that catalyzes the oxidative conversion of δ5-ene-3-beta-hydroxy steroid, as well as the conversion of various ketosteroids. Defects in the gene encoding 3β-HSD are associated with classic salt wasting, genital ambiguity, hypogonadism, insulin-resistant polycystic ovary syndrome (PCOS) and an increased susceptibility to prostate cancer. Additionally, congenital deficiency of 3β-HSD activity results in a severe depletion of steroid formation which can be lethal in young children.

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References:

1. Structure/function relationships responsible for the kinetic differences between human type 1 and type 2 3beta-hydroxysteroid dehydrogenase and for the catalysis of the type 1 activity.  |  Thomas, JL., et al. 2002. J Biol Chem. 277: 42795-801. PMID: 12205101
2. Structure/function relationships responsible for coenzyme specificity and the isomerase activity of human type 1 3 beta-hydroxysteroid dehydrogenase/isomerase.  |  Thomas, JL., et al. 2003. J Biol Chem. 278: 35483-90. PMID: 12832414
3. The hormonal phenotype of Nonclassic 3 beta-hydroxysteroid dehydrogenase (HSD3B) deficiency in hyperandrogenic females is associated with insulin-resistant polycystic ovary syndrome and is not a variant of inherited HSD3B2 deficiency.  |  Carbunaru, G., et al. 2004. J Clin Endocrinol Metab. 89: 783-94. PMID: 14764797
4. YY1 binding within the human HSD3B2 gene intron 1 is required for maximal basal promoter activity: identification of YY1 as the 3beta1-A factor.  |  Foti, DM. and Reichardt, JK. 2004. J Mol Endocrinol. 33: 99-119. PMID: 15291746
5. Serine 124 completes the Tyr, Lys and Ser triad responsible for the catalysis of human type 1 3beta-hydroxysteroid dehydrogenase.  |  Thomas, JL., et al. 2004. J Mol Endocrinol. 33: 253-61. PMID: 15291757
6. Structure/function of human type 1 3beta-hydroxysteroid dehydrogenase: An intrasubunit disulfide bond in the Rossmann-fold domain and a Cys residue in the active site are critical for substrate and coenzyme utilization.  |  Thomas, JL., et al. 2007. J Steroid Biochem Mol Biol. 107: 80-7. PMID: 17624763
7. Human 3beta-hydroxysteroid dehydrogenase types 1 and 2: Gene sequence variation and functional genomics.  |  Wang, L., et al. 2007. J Steroid Biochem Mol Biol. 107: 88-99. PMID: 17689071
8. Association between polymorphisms in HSD3B1 and UGT2B17 and prostate cancer risk.  |  Park, JY., et al. 2007. Urology. 70: 374-9. PMID: 17826523
9. HSD3B1 as a novel trophoblast-associated marker that assists in the differential diagnosis of trophoblastic tumors and tumorlike lesions.  |  Mao, TL., et al. 2008. Am J Surg Pathol. 32: 236-42. PMID: 18223326