14-3-3 sigma抗体 (5D7) 推荐用于 human 来源的14-3-3 σ WB, IP, IF, IHC(P) 和 ELISA检测

14-3-3 σ 抗体 (5D7): sc-100638

14-3-3 σ抗体 (5D7) is rated 4.7 out of 5 by 7.
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Datasheets

    • 14-3-3 σ抗体(5D7)是小鼠单克隆IgG1 (kappa轻链),规格为100 µg/ml
    • 免疫human物种的重组14-3-3 σ
    • 推荐用于 human 来源的14-3-3 σ WB, IP, IF, IHC(P) 和 ELISA检测
    • 查看我们强烈推荐的传统抗小鼠IgG二抗的重组替代品 m-IgGκ BP-HRP (mouse IgGκ binding protein-HRP)
    • 看 pan 14-3-3 (B-8): sc-133233,可查看有标记的 14-3-3 σ 抗体,包括AC, HRP, FITC, PE, Alexa Fluor® 488, 594, 647, 680 和 790
    • 关于如何获取14-3-3 σ (5D7): sc-100638免费10 µg小样,联系我们技术服务部门 (或者您当地的代理商)了解详情。

Can sc-100638: 14-3-3 σ (5D7) monoclonal antibody be used to stain formalin-fixed, paraffin-embedded (FFPE) tissue sections?

Asked by: Cweed
Thank you for your question. Yes, sc-100638: 14-3-3 σ (5D7) is recommended for use in IHC with paraffin-embedded sections. We recommend performing antigen retrieval with sodium citrate buffer (pH 6) and heat. The full protocol can be found here: https://www.scbt.com/scbt/resources/protocols/immunoperoxidase-staining
Answered by: Technical Support
Date published: 2017-03-24
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Rated 4 out of 5 by from Good antibody for WB Worked well for western blotting with MCF7 cell line
Date published: 2020-07-01
Rated 5 out of 5 by from Great Choice for WB crisp strong and clean band in Western Blot applications. Great choice for detecting 14-3-3 sigma in WB.
Date published: 2017-01-26
Rated 5 out of 5 by from WB: works well with A WB: works well with A-431 whole cell lysate -SCBT QC
Date published: 2015-04-01
Rated 5 out of 5 by from Published IF/IHC data using 5D7 on human SW480 Published IF/IHC data using 5D7 on human SW480-ADH and HT29 cells. -SCBT Publication Review
Date published: 2015-02-27
Rated 4 out of 5 by from Clean immunohistochemical stain on paraffin Clean immunohistochemical stain on paraffin embedded human tumor tissue sections. -SCBT Publication Review
Date published: 2015-02-26
Rated 5 out of 5 by from Superb immunoperoxidase nuclear and cytoplasmic Superb immunoperoxidase nuclear and cytoplasmic staining in formalin-fixed, paraffin-embedded human uterine cervix tissue. -SCBT QC
Date published: 2015-02-20
Rated 5 out of 5 by from Clean Clean, strong band observed in Western blot analysis of 14-3-3 expression in HeLa nuclear extract. -SCBT QC
Date published: 2015-01-26
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